Histone 3 anticorps (H3K4me3)
-
- Antigène Voir toutes Histone 3 (H3) Anticorps
- Histone 3 (H3)
-
Épitope
- H3K4me3
-
Reactivité
- Humain, Saccharomyces cerevisiae
-
Hôte
- Lapin
-
Clonalité
- Polyclonal
-
Conjugué
- Cet anticorp Histone 3 est non-conjugé
-
Application
- Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Immunocytochemistry (ICC), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)
- Purification
- Protein A Chromatography
- Immunogène
- This Histone H3 trimethylLys4 antibody was raised against a peptide including trimethyllysine 4 of histone H3.
- Isotype
- IgG
- Top Product
- Discover our top product H3 Anticorps primaire
-
-
- Indications d'application
-
Recommended starting concentrations are
ChIP: 3 µg per ChIP
ChIP-Seq: 4 µg each
ICC/IF: 1 - 2 µg/mL dilution
WB: 2 - 4 µg/mL dilution
CUT&RUN: 1:100
Optimal working dilution should be determined by the investigator. - Restrictions
- For Research Use only
-
- Concentration
- 1 μg/μL
- Buffer
- Purified IgG in PBS ( pH 7.5) with 30 % glycerol and 0.035 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
-
Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- Stock
- -20 °C
- Stockage commentaire
- Antibodies in solution can be stored at -20 °C for 2 years.
- Date de péremption
- 6 months
-
-
H3 ubiquitination by NEDD4 regulates H3 acetylation and tumorigenesis." dans: Nature communications, Vol. 8, pp. 14799, (2017) (PubMed).
: "Reduced expression of SET7/9, a histone mono-methyltransferase, is associated with gastric cancer progression." dans: Oncotarget, Vol. 7, Issue 4, pp. 3966-83, (2016) (PubMed).
: "Alterations in nuclear structure promote lupus autoimmunity in a mouse model." dans: Disease models & mechanisms, Vol. 9, Issue 8, pp. 885-97, (2016) (PubMed).
: "The long noncoding RNA HOTAIR has tissue and cell type-dependent effects on HOX gene expression and phenotype of urothelial cancer cells." dans: Molecular cancer, Vol. 14, pp. 108, (2015) (PubMed).
: "A balancing act: heterochromatin protein 1a and the Polycomb group coordinate their levels to silence chromatin in Drosophila." dans: Epigenetics & chromatin, Vol. 8, pp. 17, (2015) (PubMed).
: "Sex Specification and Heterogeneity of Primordial Germ Cells in Mice." dans: PLoS ONE, Vol. 10, Issue 12, pp. e0144836, (2015) (PubMed).
: "Loss of histone H3 methylation at lysine 4 triggers apoptosis in Saccharomyces cerevisiae." dans: PLoS genetics, Vol. 10, Issue 1, pp. e1004095, (2014) (PubMed).
: "The 3D tissue microenvironment modulates DNA methylation and E-cadherin expression in squamous cell carcinoma." dans: Epigenetics, Vol. 7, Issue 1, pp. 34-46, (2012) (PubMed).
: "
-
H3 ubiquitination by NEDD4 regulates H3 acetylation and tumorigenesis." dans: Nature communications, Vol. 8, pp. 14799, (2017) (PubMed).
-
- Antigène
- Histone 3 (H3)
- Autre désignation
- Histone H3 (H3 Produits)
- Synonymes
- anticorps H-3, anticorps histocompatibility 3, anticorps H3
- Poids moléculaire
- 17 kDa
- ID gène
- 3020
-