NF-kB p65 anticorps (AA 526-539)

N° du produit ABIN2668795

Il existe 1 publication pour ce produit. L’anticorps anti-NF-kB p65 Monoclonal Souris est utilisé pour la détection de NF-kB p65 dans des échantillons de Humain et Souris. Il a été validé pour WB et ChIP.

Aperçu rapide pour NF-kB p65 anticorps (AA 526-539) (ABIN2668795)

Antigène: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NF-kB p65 est non-conjugé

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Clone: 112A1021

Détail du produit anti-NF-kB p65 anticorps

Épitope: AA 526-539

Fonction: NFkB p65 antibody (mAb) (Clone 112A1021)

Purification: Affinity Purified

Immunogène: This NFkB p65 antibody was raised against a synthetic peptide corresponding to amino acid residues 526-539 of human NFkB p65.

Isotype: IgG1

Information d'application

Indications d'application: WB: 2 - 5 μg/mL dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1 % Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 μg/μL

Buffer: PBS containing 0.05 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: 4 °C,-20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Store at 4°C for short term. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-NF-kB p65 (ABIN2668795)

Sun, Alkhoury, Wang, Foster, Radecke, Tam, Edwards, Facciotti, Armstrong, Knowlton, Newman, Passerini, Simon: "IRF-1 and miRNA126 modulate VCAM-1 expression in response to a high-fat meal." dans: Circulation research, Vol. 111, Issue 8, pp. 1054-64, (2012) (PubMed).

Détails sur NF-kB p65

Antigène: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Autre désignation: NFkappaB p65

Sujet: NFkB p65 (Nuclear factor NF-kappa-B p65 subunit also known as NFkB p65 or RelA) is a member of the Rel family of transcription factors that are involved in processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. The Rel family includes c-Rel, RelA (NFkB p65, NFkB3) and RelB (I-Rel). Rel proteins form dimers with the NFkB p50 (proteolytically processed from p105/NFkB1) and p52 (proteolytically processed from p100/NFkB2) members of the NFkB family that bind DNA and activate transcription. The NFkB p65/p50 heterodimer is the most abundant form of NFkB. NFkB signaling is negatively regulated by the sequestration of the NFkB complex in the cytoplasm by its association with the IkB family of inhibitory proteins. The IkB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFkB. The IKK complex is composed of two catalytic subunits (IKKa and IKKb) and a regulatory subunit (IKKg). Upon its activation, the IKK complex phosphorylates IkB proteins (IkBa, IkBa phospho Ser32, 36), which marks them for ubiquitination and degradation. This enables the NFkB complex to translocate to the nucleus where it regulates transcription by binding to DNA.

Poids moléculaire: 65 kDa

ID gène: 5970

NCBI Accession: NP_068810

Pathways: Signalisation NF-kappaB, Signalisation RTK, TCR Signaling, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins