ITGAL anticorps

N° du produit ABIN2688904

Détail du produit anti-ITGAL anticorps

Antigène: ITGAL (Integrin alpha-L (ITGAL))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp ITGAL est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: BD Pharmingen™

Attributs du produit: The M17/4 antibody reacts with the 180 kDa αL chain of LFA-1 (CD11a/CD18, αLβ2 integrin), a heterodimeric surface glycoprotein expressed on almost all leukocytes. CD8a+CD8b- intestinal intraepithelial T lymphocytes, which are believed to be thymus independent, do not express CD11a. LFA-1 mediates a variety of heterotypic and homotypic intercellular adhesions through interaction with ICAM-1 (CD54) and ICAM-2 (CD102), including participation in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. mAb M17/4 blocks a variety of LFA-1-mediated cells interactions in vitro, and costimulatory effects have also been described. In vivo treatment with M17/4 mAb reduces the severity of graft-versus-host reactions, prolongs allograft survival, inhibits the development of autoimmunity, and blocks substance P-induced leukocyte migration. The M17/4 and 2D7 (Cat. No. 553120) antibodies are reported to recognize different epitopes of the CD11a molecule. Immunohistochemical staining of CD11a positive cells. Frozen sections of mouse spleen were reacted with the anti-CD11a antibody. Leukocytes can be identified by the brown labeling of their cell membranes. Amplification 20X.

BD Pharmingen™ Purified Rat Anti-Mouse CD11a - Purified - Clone M17/4 - Isotype Rat IgG2a, κ - Reactivity Ms - 1 mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: C57BL/6 Mouse Splenic Secondary Cytotoxic T Lymphocytes

Clone: M17-4

Isotype: IgG2a kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 31.25 μg/mL

Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-ITGAL (ABIN2688904)

Zhao, Iwata: "Cross-linking of the TCR-CD3 complex with CD4, CD8 or LFA-1 induces an anti-apoptotic signal in thymocytes: the signal is canceled by FK506." dans: International immunology, Vol. 7, Issue 9, pp. 1387-96, (1996) (PubMed).

Springer: "Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm." dans: Cell, Vol. 76, Issue 2, pp. 301-14, (1994) (PubMed).

Harning, Pelletier, Lubbe, Takei, Merluzzi: "Reduction in the severity of graft-versus-host disease and increased survival in allogenic mice by treatment with monoclonal antibodies to cell adhesion antigens LFA-1 alpha and MALA-2." dans: Transplantation, Vol. 52, Issue 5, pp. 842-5, (1991) (PubMed).

Sanders, Vitetta: "B cell-associated LFA-1 and T cell-associated ICAM-1 transiently cluster in the area of contact between interacting cells." dans: Cellular immunology, Vol. 132, Issue 1, pp. 45-55, (1991) (PubMed).

Larson, Springer: "Structure and function of leukocyte integrins." dans: Immunological reviews, Vol. 114, pp. 181-217, (1990) (PubMed).

Sanchez-Madrid, Simon, Thompson, Springer: "Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1." dans: The Journal of experimental medicine, Vol. 158, Issue 2, pp. 586-602, (1983) (PubMed).

Springer, Davignon, Ho, Kürzinger, Martz, Sanchez-Madrid: "LFA-1 and Lyt-2,3, molecules associated with T lymphocyte-mediated killing; and Mac-1, an LFA-1 homologue associated with complement receptor function." dans: Immunological reviews, Vol. 68, pp. 171-95, (1983) (PubMed).

Détails sur ITGAL

Antigène: ITGAL (Integrin alpha-L (ITGAL))

Autre désignation: CD11a (ITGAL Produits)

Synonymes: anticorps CD11A, anticorps LFA-1, anticorps LFA1A, anticorps (p180), anticorps Cd11a, anticorps LFA-1A, anticorps Ly-15, anticorps Ly-21, anticorps CD11a, anticorps ITGAL, anticorps integrin subunit alpha L, anticorps integrin alpha L, anticorps ITGAL, anticorps Itgal

Sujet: Synonyms: Integrin αL Chain, LFA-1α

Pathways: Activated T Cell Proliferation, Integrin Complex