TNFRSF1A anticorps

N° du produit ABIN2688923

Détail du produit anti-TNFRSF1A anticorps

Antigène: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TNFRSF1A est non-conjugé

Application: Flow Cytometry (FACS)

Marque: BD Pharmingen™

Attributs du produit: The TNFR1-B1 antibody reacts with the extracellular domain of CD120A which is also known as the 55 kDa receptor for the human cytokines, tumor necrosis factor (TNF) and lymphotoxin-alpha (LT-α). This receptor is referred to as the p55 or Type I Tumor Necrosis Factor Receptor (TNFRI). TNFRI are expressed by a variety of cell lines, tumor cells, and normal cell types including T cells, monocytes, macrophages, neutrophils, endothelial cells, hepatocytes, chondrocytes, and fibroblasts. Naive B cells express very low or undetectable levels of TNFRI whereas mature erythrocytes and platelets are uniformly negative for TNFRI expression. For the generation of the MABTNFR1-B1 hybridoma, BALB/c mice were immunized with purified, full-length human TNFRI protein that was expressed by insect cells that were infected with a recombinant human TNFRI-baculovirus expression vector. MABTNFR1-B1 specifically binds to natural and recombinant truncated forms of TNFRI. Expression of cell surface TNFRI by non-activated human PBMC or RBC. Human PBMC (left panel) and red blood cells (right panel) isolated by density centrifugation (Ficoll-Paque™) were stained with MABTNFR1-B1 (0.5 μg, Cat No. 550514) followed by biotinylated goat anti-mouse Ig (0.25 μg, Cat . No. 553999) and Streptavidin Phycoerythrin (0.015 μg, Cat. No. 554061) in a three-step staining protocol to amplify immunofluorescent signals. Staining with the MABTNFR1-B1 antibody (filled histograms) is compared to staining obtained using the secondary antibody alone (open histograms). Histograms in the left panel are gated on the lymphocyte population. Note: Certain human cell lines or cell types (e.g., neutrophils, monocytes) can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors.

BD Pharmingen™ Purified Mouse Anti-Human CD120a - Purified - Clone MABTNFR1-B1 - Isotype Mouse IgG2a, κ - Reactivity Hu - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: MABTNFR1

Isotype: IgG2a kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-TNFRSF1A (ABIN2688923)

Browning, Dougas, Ngam-ek, Bourdon, Ehrenfels, Miatkowski, Zafari, Yampaglia, Lawton, Meier: "Characterization of surface lymphotoxin forms. Use of specific monoclonal antibodies and soluble receptors." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 1, pp. 33-46, (1995) (PubMed).

Wallach, Engelmann, Nophar, Aderka, Kemper, Hornik, Holtmann, Brakebusch: "Soluble and cell surface receptors for tumor necrosis factor." dans: Agents and actions. Supplements, Vol. 35, pp. 51-7, (1992) (PubMed).

Heilig, Mapara, Brockhaus, Krauth, Dörken: "Two types of TNF receptors are expressed on human normal and malignant B lymphocytes." dans: Clinical immunology and immunopathology, Vol. 61, Issue 2 Pt 1, pp. 260-7, (1991) (PubMed).

Hohmann, Remy, Brockhaus, van Loon: "Two different cell types have different major receptors for human tumor necrosis factor (TNF alpha)." dans: The Journal of biological chemistry, Vol. 264, Issue 25, pp. 14927-34, (1989) (PubMed).

Munker, DiPersio, Koeffler: "Tumor necrosis factor: receptors on hematopoietic cells." dans: Blood, Vol. 70, Issue 6, pp. 1730-4, (1988) (PubMed).

Détails sur TNFRSF1A

Antigène: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Autre désignation: CD120a (TNFRSF1A Produits)

Synonymes: anticorps CD120a, anticorps FPF, anticorps MS5, anticorps TBP1, anticorps TNF-R, anticorps TNF-R-I, anticorps TNF-R55, anticorps TNFAR, anticorps TNFR1, anticorps TNFR1-d2, anticorps TNFR55, anticorps TNFR60, anticorps p55, anticorps p55-R, anticorps p60, anticorps TNF-R1, anticorps TNF-alphaR1, anticorps TNFRI, anticorps TNFRp55, anticorps TNFalpha-R1, anticorps Tnfr-2, anticorps Tnfr1, anticorps fpf, anticorps tbp1, anticorps p55-r, anticorps tnf-r, anticorps tnfar, anticorps tnfr1, anticorps cd120a, anticorps tnfr55, anticorps tnfr60, anticorps xtnfr1, anticorps wu:fj65b10, anticorps zgc:85914, anticorps TNFR I, anticorps TNFRSF1A, anticorps TNFA, anticorps cTNF, anticorps TNF receptor superfamily member 1A, anticorps tumor necrosis factor receptor superfamily, member 1a, anticorps TNF receptor superfamily member 1A S homeolog, anticorps tumor necrosis factor, anticorps TNFRSF1A, anticorps Tnfrsf1a, anticorps tnfrsf1a.S, anticorps tnfrsf1a, anticorps TNF

Sujet: Synonyms: TNF Receptor Type I

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Hepatitis C, Ubiquitin Proteasome Pathway