CD4 anticorps (Biotin)

N° du produit ABIN2689241

Détail du produit anti-CD4 anticorps (Biotin)

Antigène: CD4

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD4 est conjugé à/à la Biotin

Application: Flow Cytometry (FACS)

Marque: BD Pharmingen™

Attributs du produit: The OX-35 clone has been reported to react with the CD4 antigen on most thymocytes, a subpopulation of mature T lymphocytes (i.e. MHC class II-restricted T cells, including most T helper cells), monocytes, macrophages, some dendritic cells, and microglia. CD4 is an antigen coreceptor on the T-cell surface that interacts with MHC class II molecules on antigen-presenting cells. It participates in T-cell activation through it's association with the T-cell receptor complex and protein tyrosine kinase lck. The OX-35 clone has been reported to bind to a different epitope of CD4 than that recognized by the W3/25 and OX-38 clones. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Two-color analysis of the expression of CD4 on rat splenic leukocytes. Lewis splenocytes were simultaneously stained with biotinylated anti-rat CD4 mAb clone OX-35 (right panel) and PE conjugated anti-rat CD3 mAb clone G4.18 (Cat. No. 554833), followed by avidin-FITC (Cat. No. 554057). The CD3-negative CD4-dim cells are the monocyte/macrophage population. Flow cytometry was performed on a BD FACScan™ flow cytometry system.

BD Pharmingen™ Biotin Mouse Anti-Rat CD4 - Biotin - Clone OX-35 - Isotype Mouse IgG2a, κ - Reactivity Rat - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Rat T-cell blasts

Clone: OX

Isotype: IgG2a kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-CD4 (Biotin) (ABIN2689241)

Liu, Zhang, Jenkins, MacPherson: "Dendritic cell heterogeneity in vivo: two functionally different dendritic cell populations in rat intestinal lymph can be distinguished by CD4 expression." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 3, pp. 1146-55, (1998) (PubMed).

Wang, Wu, Shieh, Wen, Ling: "Immunohistochemical study of amoeboid microglial cells in fetal rat brain." dans: Journal of anatomy, Vol. 189 ( Pt 3), pp. 567-74, (1997) (PubMed).

Ford, Foulcher, Goodsall, Sedgwick: "Tissue digestion with dispase substantially reduces lymphocyte and macrophage cell-surface antigen expression." dans: Journal of immunological methods, Vol. 194, Issue 1, pp. 71-5, (1996) (PubMed).

Bañuls, Alvarez, Ferrero, Zapata, Ardavin: "Cell-surface marker analysis of rat thymic dendritic cells." dans: Immunology, Vol. 79, Issue 2, pp. 298-304, (1993) (PubMed).

Janeway: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." dans: Annual review of immunology, Vol. 10, pp. 645-74, (1992) (PubMed).

Bierer, Sleckman, Ratnofsky, Burakoff: "The biologic roles of CD2, CD4, and CD8 in T-cell activation." dans: Annual review of immunology, Vol. 7, pp. 579-99, (1989) (PubMed).

Jefferies, Green, Williams: "Authentic T helper CD4 (W3/25) antigen on rat peritoneal macrophages." dans: The Journal of experimental medicine, Vol. 162, Issue 1, pp. 117-27, (1985) (PubMed).

Détails sur CD4

Antigène: CD4

Autre désignation: CD4 (CD4 Produits)

Synonymes: anticorps cd4, anticorps L3T4, anticorps Ly-4, anticorps CD4mut, anticorps W3/25, anticorps p55, anticorps CD4 molecule, anticorps carcinoembryonic antigen-related cell adhesion molecule 5, anticorps T-cell surface glycoprotein CD4, anticorps CD4 antigen, anticorps Cd4 molecule, anticorps CD4, anticorps LOC100125525, anticorps cd4, anticorps Cd4

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events