CD8 alpha anticorps

N° du produit ABIN2689435

Détail du produit anti-CD8 alpha anticorps

Antigène: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD8 alpha est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Blocking Reagent (BR), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)), Immunoaffinity Chromatography (IAC)

Marque: BD Pharmingen™

Attributs du produit: The OX-8 antibody reacts with the hinge-like membrane-proximal domain of the 32 kDa α chain of the CD8 differentiation antigen. A truncated CD8 α' isoform has not been detected in the rat. The CD8 α and β chains (CD8a and CD8b, respectively) form a heterodimer on the surface of most thymocytes and a subpopulation of mature T lymphocytes (i.e., MHC class I-restricted T cells, including most T suppressor/cytotoxic cells). Intestinal intrapithelial lymphocytes, many CD8+ T cells of athymic rats, many activated CD4+ T cells, and most NK cells express CD8a without CD8b. It has been suggested that the expression of the CD8a/CD8b heterodimer is restricted to thymus-derived T lymphocytes. OX-8 antibody does not react with resting CD4+ T helper cells. CD8 is an antigen coreceptor on the T-cell surface which interacts with MHC class I molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase Ick. Macrophages have also been reported to express CD8 α and β chains, which are involved in signal transduction. Soluble OX-8 mAb partially blocks in vitro MLR and CTL activity. Immunohistochemical staining of Rat T lymphocytes. The paraffin-embedded section of normal rat spleen was stained with Purified Mouse Anti-Rat CD8a (Cat. No. 550298). CD8+ lymphocytes around the central arterioles of the white pulp are identified by the brown staining.

BD Pharmingen™ Purified Mouse Anti-Rat CD8a - Purified - Clone OX-8 - Isotype Mouse IgG1, κ - Reactivity Rat - 1 mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: High-molecular-weight rat thymocyte glycoproteins

Clone: OX

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 15.625 μg/mL

Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-CD8 alpha (ABIN2689435)

Mitnacht, Bischof, Torres-Nagel, Hünig: "Opposite CD4/CD8 lineage decisions of CD4+8+ mouse and rat thymocytes to equivalent triggering signals: correlation with thymic expression of a truncated CD8 alpha chain in mice but not rats." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 2, pp. 700-7, (1998) (PubMed).

Wallgren, Karlsson-Parra, Korsgren: "The main infiltrating cell in xenograft rejection is a CD4+ macrophage and not a T lymphocyte." dans: Transplantation, Vol. 60, Issue 6, pp. 594-601, (1995) (PubMed).

Janeway: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." dans: Annual review of immunology, Vol. 10, pp. 645-74, (1992) (PubMed).

Stitz, Sobbe, Bilzer: "Preventive effects of early anti-CD4 or anti-CD8 treatment on Borna disease in rats." dans: Journal of virology, Vol. 66, Issue 6, pp. 3316-23, (1992) (PubMed).

Torres-Nagel, Kraus, Brown, Tiefenthaler, Mitnacht, Williams, Hünig: "Differential thymus dependence of rat CD8 isoform expression." dans: European journal of immunology, Vol. 22, Issue 11, pp. 2841-8, (1992) (PubMed).

Bierer, Sleckman, Ratnofsky, Burakoff: "The biologic roles of CD2, CD4, and CD8 in T-cell activation." dans: Annual review of immunology, Vol. 7, pp. 579-99, (1989) (PubMed).

Johnson, Gagnon, Barclay, Williams: "Purification, chain separation and sequence of the MRC OX-8 antigen, a marker of rat cytotoxic T lymphocytes." dans: The EMBO journal, Vol. 4, Issue 10, pp. 2539-45, (1985) (PubMed).

Thomas, Green: "Molecular nature of the W3/25 and MRC OX-8 marker antigens for rat T lymphocytes: comparisons with mouse and human antigens." dans: European journal of immunology, Vol. 13, Issue 10, pp. 855-8, (1984) (PubMed).

Barclay: "The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues." dans: Immunology, Vol. 42, Issue 4, pp. 593-600, (1981) (PubMed).

Brideau, Carter, McMaster, Mason, Williams: "Two subsets of rat T lymphocytes defined with monoclonal antibodies." dans: European journal of immunology, Vol. 10, Issue 8, pp. 609-15, (1980) (PubMed).

Détails sur CD8 alpha

Antigène: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Autre désignation: CD8a (CD8A Produits)

Synonymes: anticorps cd8, anticorps LOC100136450, anticorps BB154331, anticorps Ly-2, anticorps Ly-35, anticorps Ly-B, anticorps Lyt-2, anticorps zgc:136643, anticorps CD8, anticorps Leu2, anticorps MAL, anticorps p32, anticorps RHACD8-4, anticorps CD8a molecule, anticorps uncharacterized LOC100125537, anticorps CD8 alpha, anticorps CD8 antigen, alpha chain, anticorps T-cell surface glycoprotein CD8 alpha chain, anticorps Cd8a, anticorps LOC100125537, anticorps LOC100136450, anticorps CD8A, anticorps cd8a, anticorps LOC100126556

Sujet: Synonyms: Cd8a, CD8α, CD8 alpha, OX-8 membrane antigen

Pathways: TCR Signaling