Cyclin E1 anticorps

N° du produit ABIN2689485

Détail du produit anti-Cyclin E1 anticorps

Antigène: Cyclin E1 (CCNE1)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cyclin E1 est non-conjugé

Application: Immunoprecipitation (IP)

Marque: BD Pharmingen™

Attributs du produit: Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes. Cyclins (regulatory subunits) bind to cdks (catalytic subunits) to form complexes that regulate the progression of the cell cycle. The main cyclin-cdks complexes formed in vertebrate cells are cyclin D-cdk4 (G0/G1), cyclin E-cdk2 (G1/S), cyclin A-cdk2 (S) and cyclin B1-cdk1 (G2/M). These complexes are regulated by activating and inhibitory phosphorylation events, as well as by interactions with small proteins that bind to cyclins, cdks, or cyclin-cdk complexes, e.g., p21 and p27[Kip1]. Specific substrates for cdk-cyclin complexes include nuclear lamins, histones, oncogenes (c-src, c-abl, SV40 large T-Ag), tumor suppressor genes (e.g., retinoblastoma protein [Rb] and p53), nucleolin, RNA polymerase II and others. Cyclin E is expressed in G1 and associates with cdk2 to form an active kinase. Cyclin E-cdk2 complexes play an important role in regulation of the G1/S restriction checkpoint in the cell cycle. Aberrant expression of cyclin E is associated with oncogenic transformation of cells. Cyclin E migrates at a reduced molecular weight of ~50 kDa.

BD Pharmingen™ Purified Mouse Anti-Human Cyclin E - Purified - Clone HE67 - Isotype Mouse IgG2b - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Recombinant human Cyclin E

Clone: HE67

Isotype: IgG2b

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-Cyclin E1 (ABIN2689485)

Keyomarsi, Herliczek: "The role of cyclin E in cell proliferation, development and cancer." dans: Progress in cell cycle research, Vol. 3, pp. 171-91, (1998) (PubMed).

Keyomarsi, OLeary, Molnar, Lees, Fingert, Pardee: "Cyclin E, a potential prognostic marker for breast cancer." dans: Cancer research, Vol. 54, Issue 2, pp. 380-5, (1994) (PubMed).

Faha, Harlow, Lees: "The adenovirus E1A-associated kinase consists of cyclin E-p33cdk2 and cyclin A-p33cdk2." dans: Journal of virology, Vol. 67, Issue 5, pp. 2456-65, (1993) (PubMed).

Sherr: "Mammalian G1 cyclins." dans: Cell, Vol. 73, Issue 6, pp. 1059-65, (1993) (PubMed).

Détails sur Cyclin E1

Antigène: Cyclin E1 (CCNE1)

Autre désignation: Cyclin E (CCNE1 Produits)

Synonymes: anticorps CCNE1, anticorps ccne, anticorps cyclinE, anticorps CCNE, anticorps AW538188, anticorps CycE1, anticorps CYCLE, anticorps Ccne, anticorps cyce2, anticorps cycE3, anticorps cyce, anticorps fa18e07, anticorps wu:fa18e07, anticorps cyclin E1, anticorps cyclin E1 L homeolog, anticorps cyclin E1 S homeolog, anticorps CCNE1, anticorps ccne1, anticorps Ccne1, anticorps ccne1.L, anticorps ccne1.S

Poids moléculaire: 50 kDa

Pathways: Cycle Cellulaire, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases