MLH1 anticorps

N° du produit ABIN2689847

Détail du produit anti-MLH1 anticorps

Antigène: MLH1 (MutL Homolog 1 (MLH1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MLH1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Marque: BD Pharmingen™

Attributs du produit: The repair of mismatched DNA is essential to maintaining the integrity of genetic information over time. In bacteria the DNA repair process is accomplished by the MutL, MutH, and MutS proteins. The MutS protein initially recognizes and binds to mismatched DNA. Following this, MutH, an endonuclease, and MutL form a complex with MutS and carry out an excision repair mechanism. When bacteria are deficient in one of these enzymes a mutator phenotype arises characterized by genetic instability. The important role played by DNA repair enzymes is emphasized by the fact that they are highly conserved from bacteria to yeast to mammals. In yeast the proteins are called MutS homolog 2 (MSH2), MutL homolog (MLH1), and PMS1 which is also a homolog of MutL. MSH2 is involved in the initial recognition of mismatched nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex it is joined by a heterodimer of MLH1 and PMS1 which together help facilitate the later steps in mismatch repair. Biochemical studies of the human homologs of DNA mismatch repair enzymes MLH1, PMS2, and MSH2 indicate that human MSH2 protein can bind mispaired DNA, and that human MLH1 and PMS2 can exist as a heterodimer. These and other studies support the conservation of eukaryotic DNA mismatch repair mechanisms. The G168-15 antibody recognizes human MLH1 (80-85 kDa). Full-length human recombinant MLH was expressed as a fusion protein, affinity purified, and used as immunogen. Immunohistochemistry of MLH-1 in human colon carcinoma. Frozen sections of human colon carcinoma were reacted with the MLH-1 antibody. Cells expressing MLH-1 can be identifed by the intense brown labeling of their cell nuclei. Magnification 20X.

BD Pharmingen™ Purified Mouse Anti-Human MLH-1 - Purified - Clone G168-15 - Isotype Mouse IgG1 - Reactivity Hu - 1 mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human recombinant MLH

Clone: G168

Isotype: IgG1

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-MLH1 (ABIN2689847)

Baker, Plug, Prolla, Bronner, Harris, Yao, Christie, Monell, Arnheim, Bradley, Ashley, Liskay: "Involvement of mouse Mlh1 in DNA mismatch repair and meiotic crossing over." dans: Nature genetics, Vol. 13, Issue 3, pp. 336-42, (1996) (PubMed).

Cleaver: "It was a very good year for DNA repair." dans: Cell, Vol. 76, Issue 1, pp. 1-4, (1994) (PubMed).

Prolla, Christie, Liskay: "Dual requirement in yeast DNA mismatch repair for MLH1 and PMS1, two homologs of the bacterial mutL gene." dans: Molecular and cellular biology, Vol. 14, Issue 1, pp. 407-15, (1994) (PubMed).

Prolla, Pang, Alani, Kolodner, Liskay: "MLH1, PMS1, and MSH2 interactions during the initiation of DNA mismatch repair in yeast." dans: Science (New York, N.Y.), Vol. 265, Issue 5175, pp. 1091-3, (1994) (PubMed).

Su, Modrich: "Escherichia coli mutS-encoded protein binds to mismatched DNA base pairs." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 14, pp. 5057-61, (1986) (PubMed).

Détails sur MLH1

Antigène: MLH1 (MutL Homolog 1 (MLH1))

Autre désignation: MLH-1 (MLH1 Produits)

Synonymes: anticorps CG11482, anticorps Dmel\\CG11482, anticorps dmlh-1, anticorps dmlh1, anticorps zgc:66301, anticorps MLH1, anticorps LOC100232198, anticorps 1110035C23Rik, anticorps AI317206, anticorps AI325952, anticorps AI561766, anticorps COCA2, anticorps FCC2, anticorps HNPCC, anticorps HNPCC2, anticorps hMLH1, anticorps mutL homolog 1, anticorps CG11482 gene product from transcript CG11482-RB, anticorps mutL homolog 1 S homeolog, anticorps mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli), anticorps DNA mismatch repair protein Mlh1, anticorps MLH1, anticorps Mlh1, anticorps mlh1.S, anticorps mlh1, anticorps LOC588545

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response