MITF anticorps

N° du produit ABIN272035

Détail du produit anti-MITF anticorps

Antigène: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MITF est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: This antibody detects endogenous levels of MEK1 protein. (region surrounding Gly174)

Réactivité croisée (Details): Species reactivity (tested):Human and Mouse.

Purification: Affinity Chromatography using epitope-specific immunogen.

Information d'application

Indications d'application: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur MITF

Antigène: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Autre désignation: MITF (MITF Produits)

Synonymes: anticorps MITF, anticorps ws2a, anticorps mitfa, anticorps MITF-A, anticorps LOC100313655, anticorps mitfb, anticorps CMM8, anticorps MI, anticorps WS2, anticorps WS2A, anticorps bHLHe32, anticorps BCC2, anticorps Bhlhe32, anticorps Gsfbcc2, anticorps Vitiligo, anticorps Wh, anticorps bw, anticorps mi, anticorps vit, anticorps MITF-H, anticorps MITF-M, anticorps CMI9, anticorps melanogenesis associated transcription factor, anticorps microphthalmia-associated transcription factor, anticorps microphthalmia-associated transcription factor S homeolog, anticorps Microphthalmia, anticorps MITF, anticorps mitf, anticorps LOC100313655, anticorps mitf.S, anticorps LOC100534525, anticorps MICROP, anticorps Mitf

Sujet: MITF (microphthalmia-associated transcription factor) is a melanocytic nuclear protein that contains basic helix-loop-helix (HLH) and leucine zipper (LZ) domains. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. MITF can directly associate with DNA as a homodimer. It is required for the development and differentiation of melanocytes. Its expression is upregulated by cAMP and cAMP dependent pathways. MITF activates several different gene promoters by binding to their E-boxes. Tyrosinase, TRP-1 and TRP-2 are pigment synthesis genes activated by MITF. When MITF is phosphorylated on Serine 73 (via the MAPK pathway), it associates with coactivators of the p300/CBP family and enhances transcription. MITF has several isoforms including MITF-M which is specifically expressed in melanocytes. In Mitfdeficient mice there is a complete absence of melanocytes.Synonyms: Mi-protein, Microphthalmia-associated transcription factor

Poids moléculaire: approx. 52 kDa

ID gène: 4286

NCBI Accession: NP_000239

UniProt: O75030

Pathways: Chromatin Binding