PKC alpha anticorps
-
- Antigène Voir toutes PKC alpha (PKCa) Anticorps
- PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))
-
Reactivité
- Humain, Souris, Rat
-
Hôte
- Lapin
-
Clonalité
- Polyclonal
-
Conjugué
- Cet anticorp PKC alpha est non-conjugé
-
Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificité
- This antibody detects endogenous levels of PKC alpha protein. (region surrounding Gly633)
- Réactivité croisée (Details)
-
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
- Pureté
- > 95 % pure by SDS-PAGE
- Top Product
- Discover our top product PKCa Anticorps primaire
-
-
- Indications d'application
-
ELISA: 1: 1000approx. 1: 5000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
-
- Concentration
- 1,0 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- DO NOT FREEZE!
- Stock
- 4 °C
- Stockage commentaire
- Store the antibody undiluted at 2-8 °C.
-
- Antigène
- PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))
- Autre désignation
- PRKCA (PKCa Produits)
- Synonymes
- anticorps pkc-alpha, anticorps pkcalpha, anticorps im:7139045, anticorps si:dkey-8l19.1, anticorps LOC100219195, anticorps AAG6, anticorps PKC-alpha, anticorps PKCA, anticorps PRKACA, anticorps AI875142, anticorps Pkca, anticorps PKRCA, anticorps protein kinase C alpha, anticorps protein kinase C, alpha L homeolog, anticorps protein kinase C, alpha, anticorps PRKCA, anticorps prkca.L, anticorps prkca, anticorps Prkca
- Sujet
- Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (alpha, betaI, betaII and gamma) and novel (n) PKC isoforms (delta, epsilon, zeta, eta and theta). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC delta and epsilon are independent of Ca2+. On the other hand, nPKC delta and epsilon, as well as all of the cPKC members, possess phorbol ester-binding activities and kinase activities.Synonyms: PKC alpha, PKC-A, PKC-alpha, PKCA, PRKACA, Protein kinase C alpha type
- Poids moléculaire
- approx. 80 kDa
- ID gène
- 5578
- NCBI Accession
- NP_002728
- UniProt
- P17252
- Pathways
- Signalisation WNT, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
-