PERK anticorps
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- Antigène Voir toutes PERK (EIF2AK3) Anticorps
- PERK (EIF2AK3) (Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3))
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp PERK est non-conjugé
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificité
- This antibody detects endogenous levels of PERK protein. (region surrounding Arg87)
- Réactivité croisée (Details)
- Species reactivity (tested):Human.
- Purification
- Affinity Chromatography using epitope-specific immunogen.
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- Discover our top product EIF2AK3 Anticorps primaire
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- Indications d'application
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ELISA: 1/40000approx. 1/60000. Western Blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50-1/200. Immunofluorescence: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.2., 15 mM Sodium Azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Antigène
- PERK (EIF2AK3) (Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3))
- Autre désignation
- EIF2AK3 (EIF2AK3 Produits)
- Synonymes
- anticorps EIF2AK3, anticorps AI427929, anticorps Pek, anticorps Perk, anticorps PEK, anticorps PERK, anticorps WRS, anticorps eukaryotic translation initiation factor 2 alpha kinase 3, anticorps EIF2AK3, anticorps Eif2ak3
- Sujet
- An interferon-inducible, RNA-dependent protein serine /threonine kinase (PKR) has been described. PKR in earlier literature is variously known as DAI, dsJ, PI kinase, p65, p67 or TIK for the mouse kinase, and p68 or p69 for the human kinase. The PKR kinase substrate is the alpha subunit of protein synthesis initiation factor eIF-2. Phosphorylation of eIF-2alpha on serine-51 results in inhibition of translation. The serine /threonine kinase catalytic domains map to the carboxy terminal half of the protein while the RNA-binding domains are located in the amino terminal region. PERK is a type I transmembrane protein located in the endoplasmic reticulum (ER) that contains a kinase domain similar to the kinase domain of PKR. PERK is activated in response to ER stress and phosphorylates eIF-2alpha, thus inhibiting the translation of mRNA.Synonyms: Eukaryotic translation initiation factor 2-alpha kinase 3, HsPEK, PEK, PERK, PRKR-like endoplasmic reticulum kinase, Pancreatic eIF2-alpha kinase
- Poids moléculaire
- approx. 125 kDa
- ID gène
- 9451
- NCBI Accession
- NP_004827
- UniProt
- Q9NZJ5
- Pathways
- Hormone Transport, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Unfolded Protein Response
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