NF-kB p65 anticorps (Middle Region)

N° du produit ABIN2779331

Cet anticorps anti-NF-kB p65 est un anticorps Lapin Polyclonal détectant NF-kB p65 dans WB et IHC. Adapté pour Humain, Souris, Rat, Boeuf (Vache), Porc et Chien.

Aperçu rapide pour NF-kB p65 anticorps (Middle Region) (ABIN2779331)

Antigène: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Porc, Chien

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NF-kB p65 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-NF-kB p65 anticorps

Épitope: Middle Region

Séquence: LVTGAQRPPD PAPAPLGAPG LPNGLLSGDE DFSSIADMDF SALLSQISS

Homologie: Cow: 100%, Dog: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%

Attributs du produit: This is a rabbit polyclonal antibody against RELA. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the middle region of human RELA

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 551 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Détails sur NF-kB p65

Antigène: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Autre désignation: RELA

Sujet: P65 is a subunit of the nuclear factor kappa-B, a second messenger, which activates the transcription of a number of genes in multiple tissues. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-1760 M62399.1 8-1767
Alias Symbols: MGC131774, NFKB3, p65
Protein Interaction Partner: GAN, CCND1, ABCA1, UBC, TCF4, SRSF7, RELA, REL, PRKDC, PLG, PEX1, NFKB2, NFKB1, MUC3A, KRT5, REV1, TXN2, FBXW11, IL1RN, IKBKB, HSPA5, HDAC1, EP300, CREBBP, MAP3K8, CHEK1, CDKN2A, CDK4, SPATA31A3, POTEJ, POTEE, PIWIL4, DIS3L2, DCD, IKBKG, CHUK, PLA2G4A, ST
Protein Size: 551

Poids moléculaire: 60 kDa

ID gène: 5970

NCBI Accession: NM_021975, NP_068810

UniProt: Q04206

Pathways: Signalisation NF-kappaB, Signalisation RTK, TCR Signaling, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins