NF-kB p65 anticorps (Center)

N° du produit ABIN2855287

Cet anticorps Lapin Polyclonal détecte spécifiquement NF-kB p65 dans WB, IF, IHC (p), ICC et ChIP. Il présente une réactivité envers Humain et Souris et a été mentionné dans 6+ publications.

Aperçu rapide pour NF-kB p65 anticorps (Center) (ABIN2855287)

Antigène: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NF-kB p65 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

Détail du produit anti-NF-kB p65 anticorps

Épitope: Center

Réactivité croisée: Boeuf (Vache), Porc, Rat (Rattus)

Réactivité croisée (Details): Pig (98 %), Rat (99 %), Bovine (98 %)

Attributs du produit: Rabbit Polyclonal antibody to NFkB p65 (v-rel reticuloendotheliosis viral oncogene homolog A (avian))
NFkB p65 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the center region of human NFkB p65. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: Suggested dilution Reference ChIP assay Assay-dependent dilution ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceChIP assayAssay-dependent dilution ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:10000*

Commentaires:

Positive Control: A431 , HepG2 , human NFkB p65-transfected 293T

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.03 mg/mL

Buffer: 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Références pour anticorps anti-NF-kB p65 (ABIN2855287)

Arellano Buendía, Tostado González, Sánchez Reyes, García Arroyo, Argüello García, Tapia, Sánchez Lozada, Osorio Alonso: "Immunomodulatory Effects of the Nutraceutical Garlic Derivative Allicin in the Progression of Diabetic Nephropathy." dans: International journal of molecular sciences, Vol. 19, Issue 10, (2019) (PubMed).

Chen, Kuo, Liao, Lin, Tien, Huang: "Silencing of MUC20 suppresses the malignant character of pancreatic ductal adenocarcinoma cells through inhibition of the HGF/MET pathway." dans: Oncogene, Vol. 37, Issue 46, pp. 6041-6053, (2019) (PubMed).

Menicacci, Cipriani, Margheri, Mocali, Giovannelli: "Modulation of the Senescence-Associated Inflammatory Phenotype in Human Fibroblasts by Olive Phenols." dans: International journal of molecular sciences, Vol. 18, Issue 11, (2018) (PubMed).

Chamard-Jovenin, Thiebaut, Chesnel, Bresso, Morel, Smail-Tabbone, Devignes, Boukhobza, Dumond: "Low-Dose Alkylphenol Exposure Promotes Mammary Epithelium Alterations and Transgenerational Developmental Defects, But Does Not Enhance Tumorigenic Behavior of Breast Cancer Cells." dans: Frontiers in endocrinology, Vol. 8, pp. 272, (2017) (PubMed).

Thiebaut, Chamard-Jovenin, Chesnel, Morel, Djermoune, Boukhobza, Dumond: "Mammary epithelial cell phenotype disruption in vitro and in vivo through ERalpha36 overexpression." dans: PLoS ONE, Vol. 12, Issue 3, pp. e0173931, (2017) (PubMed).

Wu, Hwang-Verslues, Lee, Huang, Wei, Chen, Shew, Lee, Jeng, Tien, Ma, Lee: "Targeting IL-17B-IL-17RB signaling with an anti-IL-17RB antibody blocks pancreatic cancer metastasis by silencing multiple chemokines." dans: The Journal of experimental medicine, Vol. 212, Issue 3, pp. 333-49, (2015) (PubMed).

Détails sur NF-kB p65

Antigène: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Autre désignation: NFkB p65

Sujet: NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).[supplied by OMIM]

Cellular Localization: Nucleus , Cytoplasm

Poids moléculaire: 60 kDa

ID gène: 5970

Pathways: Signalisation NF-kappaB, Signalisation RTK, TCR Signaling, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins