ATG5 anticorps (N-Term) (Alkaline Phosphatase (AP))

N° du produit ABIN2868891

Détail du produit anti-ATG5 anticorps (Alkaline Phosphatase (AP))

Antigène: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Épitope: N-Term

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ATG5 est conjugé à/à la Alkaline Phosphatase (AP)

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificité: Predicted molecular weight at ~32.4 kDa. Observed molecular weights at ~48-60 kDa based on ATG12-ATG5 heterodimer.

Réactivité croisée: Humain

Purification: Peptide Affinity Purified

Immunogène: Synthetic peptide from the N-terminal of human ATG5

Information d'application

Indications d'application:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Commentaires:

A 1:1000 dilution of ABIN2868891 was sufficient for detection of ATG5 on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Conjugated antibodies should be stored at 4°C

Détails sur ATG5

Antigène: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Autre désignation: ATG5 (ATG5 Produits)

Synonymes: anticorps APG5, anticorps APG5-LIKE, anticorps APG5L, anticorps ASP, anticorps hAPG5, anticorps ATG5, anticorps CG1643, anticorps DmAtg5, anticorps Dmel\\CG1643, anticorps atg5, anticorps 2010107M05Rik, anticorps 3110067M24Rik, anticorps AW319544, anticorps Apg5l, anticorps Atg5l, anticorps C88337, anticorps Paddy, anticorps apg5l, anticorps zgc:100934, anticorps ATATG5, anticorps AUTOPHAGY 5, anticorps MKP11.20, anticorps MKP11_20, anticorps autophagy related 5, anticorps Autophagy-related 5, anticorps ATG5 autophagy related 5 homolog (S. cerevisiae), anticorps autophagy related 5 L homeolog, anticorps similar to S. cerevisiae ATG5 (YPL149W) which nucleates preautophagosome formation as a conjugate with Atg12, anticorps autophagy protein Apg5 family, anticorps ATG5, anticorps Atg5, anticorps atg5, anticorps atg5.L, anticorps APG5

Sujet: ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (1). Conjugation to ATG12 is essential for autophagy, but is not required for association with isolation membrane. ATG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. ATG5 is activated by ATG7 and forms a complex with ATG12 and ATG16L1. This complex is necessary for LC3-1 conjugation to PE to form LC3-II (2).

ID gène: 9474

NCBI Accession: NP_001273035

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy