Bcl-2 anticorps (AA 41-54)
-
- Antigène Voir toutes Bcl-2 (BCL2) Anticorps
- Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))
-
Épitope
- AA 41-54
-
Reactivité
- Humain
-
Hôte
- Souris
-
Clonalité
- Monoclonal
-
Conjugué
- Cet anticorp Bcl-2 est non-conjugé
-
Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
- Aucune reactivité croisée
- Souris, Rat (Rattus)
- Purification
- Protein G purification
- Immunogène
- A synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG) of the human protein was used as the immunogen for both antibodies in this Bcl2 antibody cocktail.
- Clone
- 100-D5-124
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product BCL2 Anticorps primaire
-
-
- Indications d'application
-
Variations in protocols, secondaries and substrates may require the antibody cocktail to be titered for optimal performance.
1. FFPE staining is enhanced by boiling tissue sections in 1 mM EDTA Buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow: 0.5-1 μg/million cells,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunoprecipitation: 0.5-1 μg/500 μg protein lysate,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) - Restrictions
- For Research Use only
-
- Concentration
- 0.2 mg/mL
- Buffer
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Store the Bcl2 antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
-
- Antigène
- Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))
- Autre désignation
- Bcl2 (BCL2 Produits)
- Synonymes
- anticorps Bcl-2, anticorps PPP1R50, anticorps bcl-2, anticorps AW986256, anticorps C430015F12Rik, anticorps D630044D05Rik, anticorps D830018M01Rik, anticorps BCL-2, anticorps Bcl2, anticorps BCL2, apoptosis regulator, anticorps B cell leukemia/lymphoma 2, anticorps B-cell CLL/lymphoma 2a, anticorps B-cell CLL/lymphoma 2 protein, anticorps BCL2, anticorps Bcl2, anticorps bcl2a, anticorps BCL-2
- Sujet
- B-cell lymphoma 2 is an oncogene that regulates apoptosis, having both inducing and inhibiting functions. In cancer cells, Bcl2 binds pro-apoptotic proteins, preventing programmed cell death from occurring. Overexpression is seen in many cancers including lung, breast, prostate and lymphomas. In most follicular lymphomas, neoplastic germinal centers express high levels of the protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, Bcl2 antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies.
- ID gène
- 596
- Pathways
- Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
-