CDC20 anticorps
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- Antigène Voir toutes CDC20 Anticorps
- CDC20 (Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CDC20 est non-conjugé
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purification
- Protein G purified antibody
- Immunogène
- Denatured recombinant human protein was used as the immunogen for this Cdc20 antibody.
- Clone
- AR12
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CDC20 Anticorps primaire
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- Indications d'application
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Cdc20 antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2) - Restrictions
- For Research Use only
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- Concentration
- 0.2 mg/mL
- Buffer
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Store the Cdc20 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Antigène
- CDC20 (Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))
- Autre désignation
- Cdc20 (CDC20 Produits)
- Synonymes
- anticorps X-FZY, anticorps ba276h19.3, anticorps cdc20a, anticorps fizzy1, anticorps p55cdc, anticorps CDC20A, anticorps bA276H19.3, anticorps p55CDC, anticorps 2310042N09Rik, anticorps C87100, anticorps cell division cycle 20, anticorps cell division cycle 20 L homeolog, anticorps cell division cycle 20 homolog (S. cerevisiae), anticorps cdc20, anticorps CDC20, anticorps cdc20.L, anticorps Cdc20
- Sujet
- Cyclins, regulatory subunits which associate with kinases, control many of the important steps in cell cycle progression. The Cdc2 kinase (p34Cdc2) exhibits kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested.
- ID gène
- 991
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