NGFR anticorps

N° du produit ABIN3025048

Détail du produit anti-NGFR anticorps

Antigène: NGFR (Nerve Growth Factor Receptor (NGFR))

Reactivité: Humain, Lapin, Cat, Furet, Singe, Babouin

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NGFR est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Aucune reactivité croisée: Souris, Rat (Rattus)

Attributs du produit: It recognizes a glycoprotein of 75 kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). Its epitope spans in aa 1-160 of extracellular domain of NGFR/NTR. NGF-receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. NGF is important for the development, differentiation, and survival of variety of neuronal and non-neuronal cells. Its action is mediated by binding two distinct receptors, the high affinity p140 and low affinity p75.

Purification: Protein G affinity chromatography

Immunogène: NGFR from A875 melanoma cells was used as the immunogen for the p75NTR antibody.

Clone: NGFR5

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal dilution of the p75NTR antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Prediluted IHC only format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Prediluted in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide, *For IHC use only*

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the p75NTR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur NGFR

Antigène: NGFR (Nerve Growth Factor Receptor (NGFR))

Autre désignation: p75NTR (NGFR Produits)

Synonymes: anticorps ngfr, anticorps wu:fb38c02, anticorps wu:fc10h01, anticorps CD271, anticorps Gp80-LNGFR, anticorps TNFRSF16, anticorps p75(NTR), anticorps p75NTR, anticorps LNGFR, anticorps RNNGFRR, anticorps Tnfrsf16, anticorps p75, anticorps NRH1b, anticorps fullback, anticorps nrh1, anticorps p75ntr, anticorps tnfrsf16, anticorps p75NGFR, anticorps nerve growth factor receptor, anticorps neurotrophin receptor associated death domain, anticorps nerve growth factor receptor S homeolog, anticorps nerve growth factor receptor (TNFR superfamily, member 16), anticorps NGFR, anticorps nradd, anticorps Ngfr, anticorps ngfr.S

Sujet: It recognizes a glycoprotein of 75  kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). Its epitope spans in aa 1-160 of extracellular domain of NGFR/NTR. NGF-receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. NGF is important for the development, differentiation, and survival of variety of neuronal and non-neuronal cells. Its action is mediated by binding two distinct receptors, the high affinity p140 and low affinity p75.

Pathways: Signalisation NF-kappaB, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding