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Cyclin D1 anticorps

CCND1 Reactivité: Humain WB, FACS, IF, IHC (p) Hôte: Souris Monoclonal CCND1-809 unconjugated
N° du produit ABIN3025830
  • Antigène Voir toutes Cyclin D1 (CCND1) Anticorps
    Cyclin D1 (CCND1)
    Reactivité
    • 234
    • 131
    • 124
    • 38
    • 19
    • 19
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    • 4
    • 3
    • 3
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    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 201
    • 57
    • 1
    Souris
    Clonalité
    • 193
    • 64
    Monoclonal
    Conjugué
    • 116
    • 28
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    • 2
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    • 2
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    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Cyclin D1 est non-conjugé
    Application
    • 197
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    Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Purification
    Protein G affinity chromatography
    Immunogène
    Recombinant human protein was used as the immunogen for the Cyclin D1 antibody.
    Clone
    CCND1-809
    Isotype
    IgG2a kappa
    Top Product
    Discover our top product CCND1 Anticorps primaire
  • Indications d'application
    Optimal dilution of the Cyclin D1 antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    Agent conservateur
    Azide free
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Store the Cyclin D1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Antigène
    Cyclin D1 (CCND1)
    Autre désignation
    Cyclin D1 (CCND1 Produits)
    Synonymes
    anticorps BCL1, anticorps D11S287E, anticorps PRAD1, anticorps U21B31, anticorps AI327039, anticorps CycD1, anticorps Cyl-1, anticorps bcl-1, anticorps cD1, anticorps cb161, anticorps cycd1, anticorps etID37810.7, anticorps fb52e01, anticorps fc45c08, anticorps fc83a12, anticorps id:ibd1198, anticorps wu:fb52e01, anticorps wu:fc45c08, anticorps wu:fc83a12, anticorps ccnd1b, anticorps prad1, anticorps ccnd1, anticorps ccnd1a, anticorps bcl1, anticorps d11s287e, anticorps u21b31, anticorps CCND1, anticorps cyclin D1, anticorps cyclin D1 S homeolog, anticorps cyclin D1 L homeolog, anticorps CCND1, anticorps Ccnd1, anticorps ccnd1, anticorps ccnd1.S, anticorps ccnd1.L
    Sujet
    Recognizes a protein of 36  kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60 % of mantle cell lymphomas (MCL) contain a t(11, 14)(q13, q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.
    Pathways
    Signalisation PI3K-Akt, Cycle Cellulaire, Mitotic G1-G1/S Phases, ER-Nucleus Signaling
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