TdT anticorps (AA 316-509)

N° du produit ABIN3030715

Détail du produit anti-TdT anticorps

Antigène: TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

Épitope: AA 316-509

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TdT est non-conjugé

Application: Western Blotting (WB)

Purification: Antigen affinity

Immunogène: Human partial recombinant protein (AA 316-509) was used as the immunogen for this TdT antibody.

Isotype: IgG

Information d'application

Indications d'application: The stated application concentrations are suggested starting amounts. Titration of the TdT antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Restrictions: For Research Use only

Stockage

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Stock: -20 °C

Stockage commentaire: After reconstitution, the TdT antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Détails sur TdT

Antigène: TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

Autre désignation: TdT (DNTT Produits)

Synonymes: anticorps TdT, anticorps TDT, anticorps BB160593, anticorps Tdt, anticorps dntt-A, anticorps dntt, anticorps DNA nucleotidylexotransferase, anticorps deoxynucleotidyltransferase, terminal, anticorps DNA nucleotidylexotransferase L homeolog, anticorps terminal deoxynucleotidyl transferase, anticorps DNTT, anticorps dntt, anticorps Dntt, anticorps dntt.L, anticorps tdt

Sujet: Terminal Deoxynucleotidyl Transferase, also known as Terminal transferase, is a unique DNA polymerase that without template direction catalyzes the addition of deoxyribonucleotides onto the 3-prime-hydroxyl end of DNA primers. Its gene is mapped to the region 10q23-q24. And TdT cDNA contains an open reading frame of 1,530 basepairs corresponding to a protein containing 510 amino acids. TdT may be responsible for inserting nucleotides (N regions) at the V(H)-D and D-J(H) junctions of immunoglobulin genes. The enzyme is present in immature thymocytes, some bone marrow cells, transformed pre-B and pre-T cell lines, and leukemia cells. Additionally, TdT catalyses the addition of nucleotides to the 3' terminus of a DNA molecule. Unlike most DNA polymerases it does not require a template. The preferred substrate of this enzyme is a 3'-overhang, but it can also add nucleotides to blunt or recessed 3' ends. Cobalt is a necessary cofactor.

ID gène: 1791

Pathways: Réparation de l'ADN