PKC alpha anticorps (N-Term)

N° du produit ABIN3043025

Détail du produit anti-PKC alpha anticorps

Antigène: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Épitope: AA 153-182, N-Term

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PKC alpha est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for Protein kinase C alpha type(PRKCA) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Séquence: MDHTEKRGRI YLKAEVADEK LHVTVRDAKN

Réactivité croisée (Details): Predicted Cross Reactivity: mouse, rat
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Attributs du produit: Rabbit IgG polyclonal antibody for Protein kinase C alpha type(PRKCA) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: protein kinase C, alpha
Protein Name: Protein kinase C alpha type

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminus of human PRKCA (153-182aa MDHTEKRGRIYLKAEVADEKLHVTVRDAKN), different from the related rat and mouse sequences by one amino acid.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Références pour anticorps anti-PKC alpha (ABIN3043025)

Yan-Fang, Zhi-Heng, Li-Xiao, Fang, Jun, Gang, Lan, Na-Na, Xiao-Juan, Li-Chao, Wen-Li, Pei-Fang, He, Guang-Hao, Yan-Hong, Yi-Ping, Yun-Yun, Hui-Ting, Yi, Mei-Fang, Lin, Jian, Shao-Yan, Xue-Ming, Xing et al.: "Molecular Mechanism of the Cell Death Induced by the Histone Deacetylase Pan Inhibitor LBH589 (Panobinostat) in Wilms Tumor Cells. ..." dans: PLoS ONE, Vol. 10, Issue 7, pp. e0126566, (2016) (PubMed).

Gan, Wang, Zhao, Wu, Yang, Peng: "5-(Bis(3-(2-hydroxyethyl)-1H-indol-2-yl)methyl)-2-hydroxybenzoic acid (BHIMHA): showing a strategy of designing drug to block lung metastasis of tumors." dans: Drug design, development and therapy, Vol. 10, pp. 711-21, (2016) (PubMed).

Cheng, Gu, Yuan, Feng, Jia: "Suppression of A549 cell proliferation and metastasis by calycosin via inhibition of the PKC-α/ERK1/2 pathway: An in vitro investigation." dans: Molecular medicine reports, Vol. 13, Issue 4, pp. 3709-10, (2016) (PubMed).

Sun, Li, Ma, Liu, Xu, Ye, Hou, Wang, Li, Jiang: "The predictive value and potential mechanisms of miRNA-328 and miRNA-378 for brain metastases in operable and advanced non-small-cell lung cancer." dans: Japanese journal of clinical oncology, Vol. 45, Issue 5, pp. 464-73, (2015) (PubMed).

Cheng, Gu, Zhang, Yuan, Zhao, Jiang, Jia: "Astragaloside IV inhibits migration and invasion in human lung cancer A549 cells via regulating PKC-?-ERK1/2-NF-?B pathway." dans: International immunopharmacology, Vol. 23, Issue 1, pp. 304-13, (2015) (PubMed).

Détails sur PKC alpha

Antigène: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Autre désignation: PRKCA (PKCa Produits)

Synonymes: anticorps pkc-alpha, anticorps pkcalpha, anticorps im:7139045, anticorps si:dkey-8l19.1, anticorps LOC100219195, anticorps AAG6, anticorps PKC-alpha, anticorps PKCA, anticorps PRKACA, anticorps AI875142, anticorps Pkca, anticorps PKRCA, anticorps protein kinase C alpha, anticorps protein kinase C, alpha L homeolog, anticorps protein kinase C, alpha, anticorps PRKCA, anticorps prkca.L, anticorps prkca, anticorps Prkca

Sujet: Protein kinase C(PKC) is the major phorbol ester receptor. Activation of PKC by calcium ions and the second messenger diacylglycerol is though to play a central role in the induction of cellular responses to a variety of ligand-receptor systems and in the regulation of cellular responsiveness to external stimuli. Three of these, termed alpha, beta and gamma, are highly homologous. PRKCA1 is mapped to 17q22-q23.2. PKC-alpha regulates cardiac contractility and propensity toward heart failure.

Synonyms: AAG6 antibody|Aging associated gene 6 antibody|KPCA_HUMAN antibody|MGC129900 antibody|MGC129901 antibody|PICK 1 antibody|PKC A antibody|PKC alpha antibody|PKC alpha antibody|PKC-A antibody|PKC-alpha antibody|PKCA antibody|PRKACA antibody|PRKC A antibody|PRKCA antibody|Protein Kinase C alpha antibody|Protein kinase C alpha type antibody

UniProt: P17252

Pathways: Signalisation WNT, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling