Glucocorticoid Receptor anticorps (AA 1-373)

N° du produit ABIN3043453

Détail du produit anti-Glucocorticoid Receptor anticorps

Antigène: Glucocorticoid Receptor (NR3C1) (Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1))

Épitope: AA 1-373

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Glucocorticoid Receptor est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for Glucocorticoid receptor(NR3C1) detection. Tested with WB, IHC-P in Human,Rat.

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for Glucocorticoid receptor(NR3C1) detection. Tested with WB, IHC-P in Human,Rat.
Gene Name: nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
Protein Name: Glucocorticoid receptor

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human NR3C1 recombinant protein (Position: M1-D373). Human NR3C1 shares 85% and 82% amino acid (aa) sequences identity with mouse and rat NR3C1, respectively.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for NR3C1 is approximately 0.2 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Références pour anticorps anti-Glucocorticoid Receptor (ABIN3043453)

Sharma, Maras, Das, Hussain, Mishra, Shasthry, Sharma, Weiss, Elkrief, Rautou, Gilgenkrantz, Lotersztajn, Paradis, de la Grange, Junot, Moreau, Sarin: "Pre-therapy liver transcriptome landscape in Indian and French patients with severe alcoholic hepatitis and steroid responsiveness." dans: Scientific reports, Vol. 7, Issue 1, pp. 6816, (2017) (PubMed).

Détails sur Glucocorticoid Receptor

Antigène: Glucocorticoid Receptor (NR3C1) (Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1))

Autre désignation: NR3C1 (NR3C1 Produits)

Synonymes: anticorps GR, anticorps gcr, anticorps grl, anticorps xGR, anticorps gccr, anticorps gr, anticorps zgc:113038, anticorps NR3C1, anticorps GCL, anticorps LOC100302444, anticorps Gcr, anticorps Grl, anticorps Grl-1, anticorps Grl1, anticorps GRL, anticorps GCCR, anticorps GCR, anticorps NO, anticorps GR-A, anticorps nr3c1, anticorps nuclear receptor subfamily 3 group C member 1, anticorps nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor), anticorps nuclear receptor subfamily 3, group C, member 1, anticorps glucocorticoid receptor, anticorps NR3C1, anticorps nr3c1, anticorps LOC100302444, anticorps Nr3c1

Sujet: The glucocorticoid receptor (GR, or GCR), also known as NR3C1, is the receptor to which cortisol and other glucocorticoids bind. In humans, the GR protein is encoded by NR3C1 gene which is located on chromosome 5 (5q31). GR is expressed in almost every cell in the body and regulates genes controlling the development, metabolism, and immune response. Because the receptor gene is expressed in several forms, it has many different (pleiotropic) effects in different parts of the body. The activated GR complex up-regulates the expression of anti-inflammatory proteins in the nucleus or represses the expression of pro-inflammatory proteins in the cytosol (by preventing the translocation of other transcription factors from the cytosol into the nucleus).

Synonyms: GCCR antibody|GCR antibody|GCR_HUMAN antibody|GCRST antibody|glucocorticoid nuclear receptor variant 1 antibody|Glucocorticoid receptor antibody|Glucocorticoid receptor lymphocyte antibody|GR antibody|GRL antibody|Grl1 antibody|NR3C1 antibody|Nuclear receptor subfamily 3 group C member 1 antibody|nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) antibody

ID gène: 2908

UniProt: P04150

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Regulation of Carbohydrate Metabolic Process