Actin anticorps (Actin, alpha 1, Skeletal Muscle) (AA 359-377)

Details for Product anti-ACTA1 Antibody No. ABIN3043518
Antigène
Épitope
AA 359-377, C-Term
26
23
9
8
8
4
4
3
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivité
Humain, Souris, Rat (Rattus)
212
117
115
30
21
19
14
10
9
8
8
6
6
5
5
3
3
3
2
2
2
1
1
1
1
1
1
Hôte
Lapin
138
102
Clonalité
Polyclonal
Conjugué
Cet anticorp Actin est non-conjugé
18
14
13
5
5
5
3
3
3
3
3
3
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
175
97
86
62
47
36
14
14
8
3
2
1
Options
Fonction Rabbit IgG polyclonal antibody for Actin, alpha skeletal muscle(ACTA1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Immunogène A synthetic peptide corresponding to a sequence at the C-terminus of human Actin (359-377aa ITKQEYDEAGPSIVHRKCF), identical to the related mouse and rat sequences.
Séquence ITKQEYDEAG PSIVHRKCF
Isotype IgG
Réactivité croisée (Details) No cross reactivity with other proteins.
Attributs du produit Rabbit IgG polyclonal antibody for Actin, alpha skeletal muscle(ACTA1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: actin, alpha 1, skeletal muscle
Protein Name: Actin, alpha skeletal muscle
Purification Immunogen affinity purified.
Antigène
Autre désignation ACTA1 (ACTA1 Antibody Extrait)
Sujet Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90 % sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.

Synonyms: ACTA antibody|ACTA1 antibody|Actin alpha skeletal muscle antibody|Actin antibody|actin, alpha 1, skeletal muscle 1 antibody|actin, alpha 1, skeletal muscle antibody|Actin, alpha skeletal muscle antibody|actina antibody|actine antibody|ACTS_HUMAN antibody|aktin antibody|Alpha Actin 1 antibody|Alpha skeletal muscle Actin antibody|alpha skeletal muscle antibody|alpha-actin antibody|Alpha-actin-1 antibody|ASMA antibody|CFTD antibody| CFTD1antibody|CFTDM antibody|MPFD antibody|NEM1 antibody|NEM2 antibody|NEM3 antibody| nemaline myopathy type 3 antibody
UniProt P68133
Pathways Caspase Cascade in Apoptosis, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development
Indications d'application WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for Actin is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Commentaires

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Avoid repeated freezing and thawing.
Stock 4 °C/-20 °C
Stockage commentaire At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Images (Fournisseur)
Image no. 1 for anti-Actin, alpha 1, Skeletal Muscle (ACTA1) (AA 359-377), (C-Term) antibody (ABIN3043518) Anti- ACTIN antibody, IHC(P) IHC(P): Mouse Intestine Tissue
Image no. 2 for anti-Actin, alpha 1, Skeletal Muscle (ACTA1) (AA 359-377), (C-Term) antibody (ABIN3043518) Anti- ACTIN antibody, IHC(P) IHC(P): Human Mammary Cancer Tissue
Image no. 3 for anti-Actin, alpha 1, Skeletal Muscle (ACTA1) (AA 359-377), (C-Term) antibody (ABIN3043518) Anti- ACTIN antibody, IHC(P) IHC(P): Rat Spleen Tissue
Image no. 4 for anti-Actin, alpha 1, Skeletal Muscle (ACTA1) (AA 359-377), (C-Term) antibody (ABIN3043518) Anti- ACTIN antibody, Western blotting All lanes: Anti ACTIN at 0.5ug/ml Lane 1: Rat...
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