MFGE8 anticorps (Middle Region)

N° du produit ABIN3044017

Détail du produit anti-MFGE8 anticorps

Antigène: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Épitope: AA 165-180, Middle Region

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MFGE8 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for Lactadherin(MFGE8) detection. Tested with WB, IHC-P in Human.

Séquence: NGHEFDFIHD VNKKHK

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for Lactadherin(MFGE8) detection. Tested with WB, IHC-P in Human.
Gene Name: milk fat globule-EGF factor 8 protein
Protein Name: Lactadherin

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence in the middle region of human Human Milk Fat Globule 1(165-180aa NGHEFDFIHDVNKKHK).

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for MFGE8 is approximately 0.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Date de péremption: 12 months

Références pour anticorps anti-MFGE8 (ABIN3044017)

Becker-Dreps, Choi, Stamper, Vilchez, Velasquez, Moon, Hudgens, Jiang, Permar: "Innate Immune Factors in Mothers' Breast Milk and Their Lack of Association With Rotavirus Vaccine Immunogenicity in Nicaraguan Infants." dans: Journal of the Pediatric Infectious Diseases Society, Vol. 6, Issue 1, pp. 87-90, (2018) (PubMed).

Détails sur MFGE8

Antigène: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Autre désignation: MFGE8 (MFGE8 Produits)

Synonymes: anticorps BA46, anticorps EDIL1, anticorps HMFG, anticorps HsT19888, anticorps MFG-E8, anticorps MFGM, anticorps OAcGD3S, anticorps SED1, anticorps SPAG10, anticorps hP47, anticorps ba46, anticorps edil1, anticorps oacgd3s, anticorps MGC75581, anticorps hst19888, anticorps MGC145109, anticorps MFGE8, anticorps mfge8, anticorps zgc:154157, anticorps MGC99228, anticorps AA408458, anticorps AI325141, anticorps Mfgm, anticorps P47, anticorps lactadherin, anticorps AGS, anticorps MFGME8, anticorps MFGMP-E8, anticorps im:6895406, anticorps mfge8l, anticorps zgc:110846, anticorps milk fat globule-EGF factor 8 protein, anticorps milk fat globule-EGF factor 8 protein b, anticorps milk fat globule-EGF factor 8 protein L homeolog, anticorps milk fat globule-EGF factor 8 protein a, anticorps MFGE8, anticorps mfge8, anticorps mfge8b, anticorps mfge8.L, anticorps Mfge8, anticorps mfge8a

Sujet: MFGE8(Milk Fat Globule-Egf Factor 8), also called as Lactadherin or SED1, is a protein which in humans is encoded by the MFGE8 gene. Mfge8 is secreted protein found in vertebrates, including mammals as well as birds. By fluorescence in situ hybridization, Collins et al.(1997) mapped the MFGE8 gene to chromosome 15q25. Hanayama et al.(2002) found that MFGE8 is a factor that links apoptotic cells to phagocytes. MFGE8 specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine. MFGE8, when engaged by phospholipids, bound to cells via its RGD motif. It bound particularly strongly to cells expressing alpha-V-beta-3 integrin. Bu et al.(2007) showed that Mfge8 was expressed in intestinal lamina propria macrophages in mice. Using a wound-healing assay, they showed that Mfge8 promoted migration of intestinal epithelial cells through a PKC-epsilon(PRKCE)-dependent mechanism.

Synonyms: BA 46 antibody|BA46 antibody|Breast epithelial antigen BA46 antibody|Contains Medin antibody|EDIL 1 antibody|EDIL1 antibody|HMFG antibody|hP47 antibody|HsT19888 antibody|Lactadherin antibody|Lactadherin precursor antibody|Medin antibody|MFG E8 antibody|MFG-E8 antibody|MFGE 8 antibody|MFGE8 antibody|MFGE8 protein antibody|MFGM antibody|MFGM_HUMAN antibody|Milk fat globule EGF factor 8 antibody|Milk fat globule EGF factor 8 protein antibody|Milk fat globule-EGF factor 8 antibody|O acetyl disialoganglioside synthase antibody|OAcGD3S antibody|SED1 antibody|SPAG10 antibody|Sperm associated antigen 10 antibody|Sperm surface protein hP47 antibody

UniProt: Q08431

Pathways: SARS-CoV-2 Protein Interactome