NF-kB p65 anticorps (pSer468)

N° du produit ABIN318083

L’anticorps anti-NF-kB p65 Polyclonal Lapin est utilisé pour la détection de NF-kB p65 dans des échantillons de Humain et Souris. Il a été validé pour WB, IP et IHC (p).

Aperçu rapide pour NF-kB p65 anticorps (pSer468) (ABIN318083)

Antigène: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NF-kB p65 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-NF-kB p65 anticorps

Épitope: pSer468

Specificité: This antibody detects endogenous levels of NFκB-p65 pSer468 protein.

Purification: Affinity Chromatography using epitope-specific immunogen.

Information d'application

Indications d'application: ELISA: 1/10000-1/20000. Western Blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/200. Immunoprecipitation: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2 containing 0.05 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur NF-kB p65

Antigène: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Autre désignation: RELA / NF-kB p65

Sujet: Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are members of a family of transcription factors that include the two subunits of the transcription factor NFκB (p50 and p65) and the Drosophila maternal morphogen, dorsal. Both proteins specifically bind to DNA sequences that are the same or slight variations of the 10 bp kB sequence in the immunoglobulin κ light chain enhancer. This same sequence is also present in a number of other cellular and viral enhancers. The DNA binding activity of NFκB is activated and rapidly transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct 50 kDa proteins have been described, designated p105 and p100, respectively. The p105 precursor contains p50 at its amino terminus and a C-terminal region that when expressed as a separate molecule, designated PDI, binds to p50 and regulates its activity.Synonyms: NF kappa B p65, NFKB3, NFkB p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, Rel A, Transcription factor p65

Poids moléculaire: approx. 60 kDa

ID gène: 5970

NCBI Accession: NP_001138610

UniProt: Q04206

Pathways: Signalisation NF-kappaB, Signalisation RTK, TCR Signaling, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins