KRT18 anticorps (Keratin 18)

Details for Product anti-KRT18 Antibody No. ABIN335331
Antigène
Reactivité
Humain
827
215
211
111
80
58
57
47
43
32
29
28
28
28
23
14
11
9
6
3
2
2
2
1
1
Hôte
Souris
544
313
14
7
4
2
1
Clonalité (Clone)
Monoclonal ()
Conjugué
Cet anticorp KRT18 est non-conjugé
55
43
11
11
11
11
11
11
10
8
8
8
8
8
8
8
8
8
8
8
8
8
3
3
3
3
3
2
2
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC), Western Blotting (WB)
554
360
346
330
245
196
130
86
74
69
40
30
28
18
14
11
2
1
Options
Immunogène RCK106 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a mouse immunized with cytokeratins from the human bladder carcinoma cell line T24.
Clone RCK106
Isotype IgG1
Specificité RCK106 reacts exclusively with cytokeratin 18 in glandular epithelial cells of the digestive, respiratory, and urogenital tracts, endocrine and exocrine cells and mesothelial cells, as well as adenocarcinomas originating from them.
Purification Purified
Antigène
Autre désignation Cytokeratin 18 / Keratin K18 (KRT18 Antibody Extrait)
Sujet Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
UniProt P05783
Pathways Apoptose, Caspase Cascade in Apoptosis
Indications d'application RCK106 is useful for immunocytochemistry, immunohistochemistry on frozen sections, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
Restrictions For Research Use only
Stock 4 °C
Images (Fournisseur)
Image no. 1 for anti-Keratin 18 (KRT18) antibody (ABIN335331) Immunohistochemistry on paraffin section of human colon
Produit citée dans: Bonfrer, Groeneveld, Korse, van Dalen, Oomen, Ivanyi: "Monoclonal antibody M3 used in tissue polypeptide-specific antigen assay for the quantification of tissue polypeptide antigen recognizes keratin 18." dans: Tumour biology, Vol. 15, Issue 4, pp. 210-22, 1994 (PubMed).

Bauwens, De Groot, Ramaekers, Veldman, Huizing: "Expression of intermediate filament proteins in the adult human vestibular labyrinth." dans: The Annals of otology, rhinology, and laryngology, Vol. 101, Issue 6, pp. 479-86, 1992 (PubMed).

Smedts, Ramaekers, Troyanovsky, Pruszczynski, Link, Lane, Leigh, Schijf, Vooijs: "Keratin expression in cervical cancer." dans: The American journal of pathology, Vol. 141, Issue 2, pp. 497-511, 1992 (PubMed).

Ramaekers, van Niekerk, Poels, Schaafsma, Huijsmans, Robben, Schaart, Vooijs: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." dans: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, 1990 (PubMed).

Schaafsma, Ramaekers, van Muijen, Lane, Leigh, Robben, Huijsmans, Ooms, Ruiter: "Distribution of cytokeratin polypeptides in human transitional cell carcinomas, with special emphasis on changing expression patterns during tumor progression." dans: The American journal of pathology, Vol. 136, Issue 2, pp. 329-43, 1990 (PubMed).

Smedts, Ramaekers, Robben, Pruszczynski, van Muijen, Lane, Leigh, Vooijs: "Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia." dans: The American journal of pathology, Vol. 136, Issue 3, pp. 657-68, 1990 (PubMed).

Ivanyi, Groeneveld, Van Doornewaard, Mooi, Hageman: "Keratin subtypes in carcinomas of the uterine cervix: implications for histogenesis and differential diagnosis." dans: Cancer research, Vol. 50, Issue 16, pp. 5143-52, 1990 (PubMed).

Raats, Pieper, Vree Egberts, Verrijp, Ramaekers, Bloemendal: "Assembly of amino-terminally deleted desmin in vimentin-free cells." dans: The Journal of cell biology, Vol. 111, Issue 5 Pt 1, pp. 1971-85, 1990 (PubMed).

Ramaekers, Huysmans, Schaart, Moesker, Vooijs: "Tissue distribution of keratin 7 as monitored by a monoclonal antibody." dans: Experimental cell research, Vol. 170, Issue 1, pp. 235-49, 1987 (PubMed).