Phospholipase C gamma 1 anticorps (C-Term)

N° du produit ABIN337039

Cet anticorps Lapin Polyclonal détecte spécifiquement Phospholipase C gamma 1 dans WB, IHC, IHC (p) et IP. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour Phospholipase C gamma 1 anticorps (C-Term) (ABIN337039)

Antigène: Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Phospholipase C gamma 1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Détail du produit anti-Phospholipase C gamma 1 anticorps

Épitope: C-Term

Specificité: Recognizes endogenous levels of total human PLC gamma1 protein. Does not cross-react with PLCgamma2. Species cross-reactivity: mouse and rat. MW: 155kD.

Purification: Protein A purified

Immunogène: Synthetic peptide (KLH coupled) corresponding to the C-terminal residues of human PLCgamma1.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (1:25 - 1:100), IP (1:50), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:25-1:100.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C or -20°C, Avoid freeze-thaw cycles.

Détails sur Phospholipase C gamma 1

Antigène: Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))

Autre désignation: PLCG1

Sujet: Name/Gene ID: PLCG1
Family: Phospholipase

Synonyms: PLCG1, Phospholipase C, gamma 1, Phospholipase C-148, Phosphoinositidase C, Phospholipase C-II, Inositoltrisphosphohydrolase, PLC-gamma-1, PLC1, PLC-148, PLC-II, PLC148, NCKAP3, Phospholipase C-gamma-1, PLCgamma1

ID gène: 5335

UniProt: P19174

Pathways: Signalisation RTK, Signalisation WNT, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, VEGFR1 Specific Signals, VEGF Signaling