Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (pThr34) anticorps Primary Antibody
- Western Blotting (WB)
- Specific for the ~32k DARPP-32 protein phosphorylated at Thr34. Immunolabeling is blocked by (-phosphatase treatment.
- Réactivité croisée
- Souris, Rat (Rattus)
- bovine, canine, chicken, human, non-human primates
- Antigen Affinity Purified from Pooled Serum
- Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr34 conjugated to KLH
- Autre désignation
- PPP1R1B (PPP1R1B Antibody Extrait)
- DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac (Maldve et al., 2002, Lindskog et al., 2002, Svenningsson et al., 2002). Anti-Phospho Thr34 DARPP-32 Western blot of rat caudate lysate showing specific immunolabeling of the ~32k DARPP-32 phosphorylated at Thr34 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr34 DARPP-32. The immunolabeling is completely eliminated by treatment with (-Ptase.
- Poids moléculaire
- '32 kDa
- ID gène
- Indications d'application
- Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.
- For Research Use only
- 100 μL in 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.
- -20 °C
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