BAD anticorps (pSer136)

N° du produit ABIN362001

Cité dans 2 publications. Cet anticorps anti-BAD Polyclonal Lapin (ABIN362001) détecte spécifiquement BAD dans IHC. L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour BAD anticorps (pSer136) (ABIN362001)

Antigène: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp BAD est non-conjugé

Application: Immunohistochemistry (IHC)

Détail du produit anti-BAD anticorps

Épitope: pSer136

Specificité: The antibody detects endogenous level of BAD only when phosphorylated at serine136.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène: Peptide sequence around phosphorylation site of pSer136 (S-R-S (p) -A-P) derived from Mouse BAD. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Références pour anticorps anti-BAD (ABIN362001)

Otsuki, Sugiyama, Amano, Yasui, Sakagami: "Negative regulation of NaF-induced apoptosis by Bad-CAII complex." dans: Toxicology, Vol. 287, Issue 1-3, pp. 131-6, (2011) (PubMed).

Matsuda, Takano, Kageyama, Hatakeyama, Shakunaga, Kitajima, Yamazaki, Hattori: "Silencing of caspase-8 and caspase-3 by RNA interference prevents vascular endothelial cell injury in mice with endotoxic shock." dans: Cardiovascular research, Vol. 76, Issue 1, pp. 132-40, (2007) (PubMed).

Détails sur BAD

Antigène: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Autre désignation: BAD

Sujet: The protein encoded by BAD gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.

Poids moléculaire: 23 kDa

NCBI Accession: NP_031548

UniProt: Q61337

Pathways: Signalisation MAPK, Signalisation PI3K-Akt, Signalisation RTK, Apoptose, Fc-epsilon Receptor Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events