RPS6KB1 anticorps (AA 422-426)

N° du produit ABIN362978

Détail du produit anti-RPS6KB1 anticorps

Antigène: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Épitope: AA 422-426

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RPS6KB1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Specificité: The antibody detects endogenous level of total p70 S6 Kinase protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen. Specificity/Sensitivity p70 S6 Kinase (Ab-411) antibody detects endogenous levels of total p70 S6 Kinase protein.

Immunogène: Peptide sequence around AA 422-426 (P-V-S-P-V) derived from Human p70S6k. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Détails sur RPS6KB1

Antigène: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Autre désignation: p70 S6 Kinase (RPS6KB1 Produits)

Synonymes: anticorps PS6K, anticorps S6K, anticorps S6K-beta-1, anticorps S6K1, anticorps STK14A, anticorps p70 S6KA, anticorps p70(S6K)-alpha, anticorps p70-S6K, anticorps p70-alpha, anticorps 10539, anticorps 7084, anticorps CG10539, anticorps DS6K, anticorps Dmel\\CG10539, anticorps Dp70S6k, anticorps Dp70[s6k], anticorps Dp70s6k, anticorps S6k, anticorps dS6K, anticorps dS6k, anticorps dp70[S6k], anticorps dp70s6k, anticorps dps6k, anticorps ds6k, anticorps fs(3)07084, anticorps l(3)07084, anticorps p70 S6K, anticorps p70/S6K, anticorps p70S6K, anticorps p70[S6 kinase], anticorps p70[S6K], anticorps p70[S6k], anticorps p70[S6kinase], anticorps p70s6K, anticorps s6k, anticorps s6k11, anticorps 2610318I15Rik, anticorps 4732464A07Rik, anticorps 70kDa, anticorps AA959758, anticorps AI256796, anticorps AI314060, anticorps p70/85s6k, anticorps p70s6k, anticorps p70s6k-A, anticorps p70-s6k, anticorps ps6k, anticorps rps6kb1, anticorps rps6kb1-A, anticorps s6K1, anticorps stk14a, anticorps fc51h01, anticorps wu:fc51h01, anticorps zgc:55713, anticorps ribosomal protein S6 kinase B1, anticorps Ribosomal protein S6 kinase, anticorps ribosomal protein S6 kinase, polypeptide 1, anticorps ribosomal protein S6 kinase B1 L homeolog, anticorps ribosomal protein S6 kinase B1 S homeolog, anticorps ribosomal protein S6 kinase b, polypeptide 1b, anticorps RPS6KB1, anticorps S6k, anticorps Rps6kb1, anticorps rps6kb1.L, anticorps rps6kb1.S, anticorps rps6kb1b

Sujet: RPS6KB1 phosphorylates the Ribosomal Protein-S6. Activation of RPS6KB1 requires a complex, ordered series of conformational changes and phosphorylation reactions. While the role of sequential, multi-site phosphorylation has been extensively detailed, characterization of the priming step required to initiate this cascade has remained elusive. Probably this priming process is dependent on calcium. Calcium-dependent regulation of RPS6KB1 does not specifically target Thr-229 and Thr-389, the key regulatory phosphorylation sites, rather, calcium chelation results in a global inhibition of RPS6KB1 phosphorylation. The initial calcium-dependent process is required to release an inhibitory interaction between the C- and N-termini of RPS6KB1, thus allowing phosphorylation of key domains. The priming event involves formation of a calcium-dependent protein complex that releases the interaction between the N- and C-termini. RPS6KB1 is then accessible for activation by the kinases that target the known regulatory phosphorylation sites .

Poids moléculaire: 85 kDa

NCBI Accession: NP_003152

UniProt: P23443

Pathways: Signalisation PI3K-Akt, Signalisation RTK, AMPK Signaling, Regulation of Cell Size, Skeletal Muscle Fiber Development, Feeding Behaviour, G-protein mediated Events, Smooth Muscle Cell Migration, Interaction of EGFR with phospholipase C-gamma, L'effet Warburg