SIAH1 anticorps (N-Term)

N° du produit ABIN374155

Détail du produit anti-SIAH1 anticorps

Antigène: SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))

Épitope: N-Term

Reactivité: Humain, Rat, Souris, Chien, Boeuf (Vache)

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp SIAH1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Séquence: RQTATALPTG TSKC

Specificité: This product is expected to recognize both reported isoforms (according to NP_003022.3, NP_001006611.1).

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), and Cow.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogène: Peptide from N-Terminus of the protein sequence according to NP_003022.3, NP_001006611.1 Genename: SIAH1

Information d'application

Indications d'application: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.1-0.3 μg/mL. Approx 28+30 kDa bands observed in Human Liver lysates(calculated MW of 31.0 kDa and 34.0 kDa according to NP_003022.3 and NP_001006611.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Références pour anticorps anti-SIAH1 (ABIN374155)

Donati, Marseglia, Magi, Serratì, Cencetti, Bernacchioni, Nannetti, Benelli, Brunelli, Torricelli, Cossu, Bruni: "Sphingosine 1-phosphate induces differentiation of mesoangioblasts towards smooth muscle. A role for GATA6." dans: PLoS ONE, Vol. 6, Issue 5, pp. e20389, (2011) (PubMed).

Détails sur SIAH1

Antigène: SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))

Autre désignation: SIAH1 (SIAH1 Produits)

Synonymes: anticorps SIAH, anticorps Siah1, anticorps Siah1a, anticorps id:ibd2635, anticorps zgc:56026, anticorps SIAH1A, anticorps siah, anticorps E3 ubiquitin-protein ligase Siah1, anticorps E3 ubiquitin-protein ligase siah-1, anticorps siah E3 ubiquitin protein ligase 1, anticorps siah E3 ubiquitin protein ligase 1 L homeolog, anticorps Tsp_03055, anticorps siah-1, anticorps Siah1, anticorps siah1, anticorps SIAH1, anticorps siah1.L

Sujet: Seven in absentia homolog 1 (SIAH-1) is a member of the RING-finger-containing E3 ubiquitin ligases. Alpha-synuclein and synphilin-1 are substrates of SIAH-1 , both proteins are involved in the development of Parkinson's disease (PD). Mutations in Parkin, another E3 ubiquitin ligase which ubiquinates synphilin-1 and glycosylated alpha-synuclein, have been defined as a major cause of autosomal recessive PD. The role of SIAH-1 in PD is highlighted by the fact that SIAH-1 is a component of the Lewy bodies and plays a role in apoptosis caused by nitric oxide (NO) induced oxidative stress. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) a classic glycolytic enzyme, and multi-functional protein. GAPDH plays role as a mediator for cell death. GAPDH translocates to the nucleus under a variety of stressors/conditions, most of which are associated with oxidative stress. Sequential steps lead to nuclear translocation of GAPDH during cell death, 1] a catalytic cysteine in GAPDH (C150 in rat GAPDH) is S-nitrosylated by nitric oxide (NO) which is generated from inducible nitric oxide synthase (iNOS) and/or neuronal NOS (nNOS), 2] the modified GAPDH becomes capable of binding with Siah1, an E3 ubiquitin ligase, and stabilizes it,3] the GAPDH-Siah protein complex translocates to the nucleus, dependent on Siah1's nuclear localization signal, and degrades Siah1's substrates in the nucleus, which results in cytotoxicity. GAPDH may be genetically associated with late-onset Alzheimer's disease. (-)-deprenyl, which has been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and has neuroprotective actions. The molecular action of this protection is as yet not clear.Synonyms: E3 ubiquitin-protein ligase SIAH1, HUMSIAH, Seven in absentia homolog 1, Siah-1, Siah-1a

ID gène: 6477

NCBI Accession: NP_003022

UniProt: Q8IUQ4