Dynactin 1 anticorps (C-Term)

N° du produit ABIN374269

Détail du produit anti-Dynactin 1 anticorps

Antigène: Dynactin 1 (DCTN1)

Épitope: C-Term

Reactivité: Humain, Souris, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Dynactin 1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Séquence: C-QEQLHQLHSR LIS

Specificité: This antibody is expected to recognise all reported isoforms of Dynactin subunit 1 (NP_004073.2, NP_075408.1, NP_001128512.1, NP_001128513.1).

Réactivité croisée (Details): Species reactivity (expected):Rat.
Species reactivity (tested):Human, Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogène: Peptide from C-Terminus of the protein sequence according to NP_004073.2, NP_075408.1, NP_001128512.1, NP_001128513.1. Genename: DCTN1

Information d'application

Indications d'application: Peptide ELISA: Limit dilution 1: 128000. Western Blot: 0.5-2 μg/mL. pprox 150 kDa band observed in cell lline A549 and HumanTestis lysates (calculated MW of 140 kDa according to NP_004073.2). Immunohistochemistry: 2-4 μg/mL. In paraffin embedded Human Cerebellum showspixulate staining of cytoplasm in neuronal cells. In paraffin embedded Human Testisshows cytoplasm staining in cells of the seminiferous tubules.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Dynactin 1

Antigène: Dynactin 1 (DCTN1)

Autre désignation: Dynactin Subunit 1 (DCTN1 Produits)

Synonymes: anticorps DAP-150, anticorps DP-150, anticorps P135, anticorps Chip150, anticorps DCTN1, anticorps DKFZp469C1813, anticorps AL022633, anticorps Glued, anticorps p150, anticorps p150, anticorps CG9206, anticorps DYNA_DROME, anticorps Dmel\\CG9206, anticorps FCP-D, anticorps Glu, anticorps P150, anticorps P150[Glued], anticorps p150/Glued, anticorps p150/glued, anticorps p150[Glued], anticorps p150[glued], anticorps t1, anticorps Dynactin-1, anticorps glued, anticorps dynactin subunit 1, anticorps dynactin 1, anticorps Dynactin 1, p150 subunit, anticorps dynactin subunit 1 L homeolog, anticorps DCTN1, anticorps Dctn1, anticorps DCTN1-p150, anticorps dctn1.L

Sujet: The DCTN1 gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein. Alternative splicing of this gene results in at least 2 functionally distinct isoforms: a ubiquitously expressed one and a brain-specific one. Based on its cytogenetic location, this gene is considered as a candidate gene for limb-girdle muscular dystrophy.Synonyms: 150 kDa dynein-associated polypeptide, DAP-150, DCTN1, DP-150, p135, p150-glued

ID gène: 1639

NCBI Accession: NP_004073

UniProt: Q14203

Pathways: M Phase, ER-Nucleus Signaling