IFT74 anticorps (C-Term)

N° du produit ABIN374425

Détail du produit anti-IFT74 anticorps

Antigène: IFT74 (Intraflagellar Transport Protein 74 Homolog (IFT74))

Épitope: C-Term

Reactivité: Humain, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp IFT74 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Séquence: C-KTIVDALHST SGN

Specificité: This antibody recognizes IFT74/CCDC2 at C-term.

Réactivité croisée (Details): Species reactivity (tested):Rat and Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogène: Peptide from the C Terminus of the protein sequence according to NP_001092692.1, NP_001092693.1, NP_079379.2, AAK77221.1. Genename: IFT74

Information d'application

Indications d'application: Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.3-1 μg/mL. Approx 70-75 kDa band observed in lysates of cell line HEK293(calculated MW of 69.5 kDa according to AAK77221.1, but 51.5 kDa according toNP_079379.1). The observed molecular weight corresponds to earlier findings in literaturewith different antibodies (Momeni et al, BMC Neurol.2006 Dec 13,6: 44. , PMID: 17166276). Immunohistochemistry: 5-10 μg/mL. In paraffin embedded Human Liver shows vesicular staining in cytoplasm of hepatocytes. Immunofluorescence: Successfully used at 2.5 mg/mL to stain rat cortical neurons (see

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3, 0.02 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur IFT74

Antigène: IFT74 (Intraflagellar Transport Protein 74 Homolog (IFT74))

Autre désignation: IFT74 (IFT74 Produits)

Synonymes: anticorps CCDC2, anticorps DKFZp469F1522, anticorps 1700029H06Rik, anticorps Ccdc2, anticorps Cmg1, anticorps b2b796Clo, anticorps CMG-1, anticorps CMG1, anticorps intraflagellar transport protein 74/72, anticorps intraflagellar transport 74, anticorps intraflagellar transport protein 74 homolog, anticorps IFT74, anticorps ift74, anticorps LOC100632007, anticorps LOC100649180, anticorps Ift74

Sujet: Capillary morphogenesis protein 1 (CMG1) is a 600 amino acid protein and has a calculated molecular mass of 65 kD. CMG1 contains a coiled-coil domain (which shares 24 % identity with myosin heavy chain sequences from various species) and several putative phosphorylation sites. Bell et al. (2001) determined that CMG1 was 1 of several transcripts upregulated by umbilical vein endothelial cells during capillary morphogenesis in 3-dimensional collagen matrices. RT-PCR detected highest expression in adult and fetal kidney and lower expression in adult heart, placenta, lung, liver, and pancreas, and in fetal heart, lung, and liver. Little to no expression was detected in adult brain and skeletal muscle or in fetal brain, thymus, and spleen. Transfection into epithelial tumor cells revealed targeting of fluorescent-tagged CMG1 to an intracellular vesicular compartment.Synonyms: CCDC2, CMG-1, CMG1, Capillary morphogenesis protein 1, Coiled-coil domain-containing protein 2, Intraflagellar transport protein 74 homolog

ID gène: 80173

NCBI Accession: NP_001092692

UniProt: Q96LB3

Pathways: Signalisation Hedgehog, Chromatin Binding