WNT3 anticorps (Internal Region)

N° du produit ABIN374685

Détail du produit anti-WNT3 anticorps

Antigène: WNT3 (Wingless-Type MMTV Integration Site Family, Member 3 (WNT3))

Épitope: Internal Region

Reactivité: Humain, Souris, Rat, Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp WNT3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Specificité: This antibody recognizes WNT3.

Réactivité croisée (Details): Species reactivity (expected):Rat, Canine (Dog).
Species reactivity (tested):Human, Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogène: Peptide with sequence CGRGHNTRTEKRKEK, from the internal region of the protein sequence according to NP_110380.1. Genename: WNT3

Information d'application

Indications d'application: Peptide ELISA: 1/2000. Western Blot: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur WNT3

Antigène: WNT3 (Wingless-Type MMTV Integration Site Family, Member 3 (WNT3))

Autre désignation: WNT3 (WNT3 Produits)

Synonymes: anticorps INT4, anticorps Ci-Wnt3, anticorps WNT3, anticorps int4, anticorps Xwnt3, anticorps wnt-3, anticorps wnt3l, anticorps Xwnt-3, anticorps wnt3, anticorps Int-4, anticorps Wnt-3, anticorps wnt3-A, anticorps Wnt family member 3, anticorps wingless-type MMTV integration site family, member 3, anticorps proto-oncogene Wnt-3, anticorps wingless-type MMTV integration site family member 3, anticorps Wnt family member 3A, anticorps wingless-type MMTV integration site family member 3 L homeolog, anticorps WNT3, anticorps Wnt3, anticorps wnt3, anticorps LOC468297, anticorps WNT3A, anticorps wnt3.L

Sujet: Wnts have diverse roles in governing cell fate, proliferation, migration, and polarity during development, particularly in embryogenesis and carcinogenesis. WNTs are transduced through at least three distinct intracellular signaling pathways, including the canonical Wnt/b-catenin, Wnt/Ca2+ and Wnt polarity pathways. Distinct sets of Wnt and Frizzled ligand-receptor pairs activate each of these pathways and lead to unique cellular responses. Wnt3 is expressed in embryonic mouse brain (with peak expression at around day 12 of gestation) and at low levels in the adult. It is present in the cerebellum during mouse development, and is restricted to the Purkinje cell layer in the adult mouse. Wnt3 is required for normal limb development and plays an important role in the establishment of the apical ectodermal ridge. In motorneurons, Wnt3 is a retrograde signal that controls terminal branching of muscle afferents. Wnt3 is highly expressed in human cancer cell lines including A549 (lung cancer), MKN45 (gastric cancer) and MCF7 (breast cancer). Wnt3 and Wnt3a are nearly 90 % identical at the amino acid level.Synonyms: INT-4, INT4, Proto-oncogene protein Wnt-3

ID gène: 7473, 9606

UniProt: P56703

Pathways: Signalisation WNT, Regulation of Cell Size