Patched 1 anticorps (Internal Region)

N° du produit ABIN375146

Détail du produit anti-Patched 1 anticorps

Antigène: Patched 1 (PTCH1)

Épitope: Internal Region

Reactivité: Poisson zèbre (Danio rerio)

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Patched 1 est non-conjugé

Application: Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Séquence: C-QTGSKKEPFN YSQ

Specificité: This antibody recognizes internal PTCH1.

Réactivité croisée (Details): Species reactivity (tested):Zebrafish.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide with sequence from the internal region of the protein sequence according to NP_571063.1

Information d'application

Indications d'application: Peptide ELISA: Antibody Detection Limit Dilution: 1/32000. Immunofluorescence studies of Zebrafish tumors: 1-3 μg/mL (Ju et al, Mol Cancer.2009 Jun25,8: 40. PMID: 19555497).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Patched 1

Antigène: Patched 1 (PTCH1)

Autre désignation: PTCH1 (PTCH1 Produits)

Synonymes: anticorps BCNS, anticorps HPE7, anticorps NBCCS, anticorps PTC, anticorps PTC1, anticorps PTCH, anticorps PTCH11, anticorps lep, anticorps ptc1, anticorps ptc2, anticorps Ptch, anticorps Ptch2, anticorps Ptc-1, anticorps XPtc1, anticorps patched, anticorps patched1, anticorps A230106A15Rik, anticorps Ptc, anticorps Ptc1, anticorps mes, anticorps patched 1, anticorps patched 1 L homeolog, anticorps Protein patched homolog 1, anticorps PTCH1, anticorps ptch1, anticorps Ptch1, anticorps ptch1.L, anticorps ptc-1

Sujet: PTCH (Patched protein homolog 1) is a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). PTCH associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal.PTCH has a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis. PTCH is expressed in the adult brain, lung, liver, heart, placenta, skeletal muscle, pancreas and kidney. It is also expressed in tumor cells but not in normal skin. During development PTCH is found in all major target tissues of sonic hedgehog, such as the ventral neural tube, somites, and tissues surrounding the zone of polarizing activity of the limb bud. Defects in PTCH are probably the cause of basal cell nevus syndrome also known as Gorlin syndrome or Gorlin-Goltz syndrome.Synonyms: PTC, PTC1, PTCH, Patched 1, Protein patched homolog 1

ID gène: 30181, 7955

UniProt: Q98864

Pathways: Signalisation Hedgehog, Carbohydrate Homeostasis, Tube Formation