BAD anticorps (pSer99)

N° du produit ABIN389521

Cet anticorps Lapin Polyclonal détecte spécifiquement BAD dans DB, IHC (p) et IF. Il présente une réactivité envers Humain.

Aperçu rapide pour BAD anticorps (pSer99) (ABIN389521)

Antigène: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp BAD est non-conjugé

Application: Dot Blot (DB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Clone: RB06933

Détail du produit anti-BAD anticorps

Épitope: pSer99

Homologie: M, Rat

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogène: This Bad Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S99 of human Bad.

Isotype: Ig Fraction

Information d'application

Indications d'application: IF: 1:200. IHC-P: 1:50~100. DB: 1:500

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Date de péremption: 6 months

Détails sur BAD

Antigène: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Autre désignation: Bad

Sujet: Bad is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin are found to be involved in the regulation of this protein. Bad is phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation.

Poids moléculaire: 18392

ID gène: 572

NCBI Accession: NP_004313, NP_116784

UniProt: Q92934

Pathways: Signalisation MAPK, Signalisation PI3K-Akt, Signalisation RTK, Apoptose, Fc-epsilon Receptor Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events