ECT2 anticorps (pThr790)

N° du produit ABIN401345

Détail du produit anti-ECT2 anticorps

Antigène: ECT2 (Epithelial Cell Transforming Sequence 2 Oncogene (ECT2))

Épitope: AA 785-795, pThr790

Reactivité: Humain, Souris, Rat, Chien, Poisson zèbre (Danio rerio), Poulet, Chimpanzé

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ECT2 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Specificité: This antibody is directed against the phosphorylated form of ECT2 protein at the pT790 residue.

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat, Zebrafish, Chimpanzee, Chicken, Canine.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogène: Synthetic peptide corresponding to amino acids 785-795 of human ECT2 protein

Isotype: IgG

Information d'application

Indications d'application: ELISA: 1/10,000 - 1/40,000. Western Blot: 1/1,000 - 1/4,000. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.31 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Détails sur ECT2

Antigène: ECT2 (Epithelial Cell Transforming Sequence 2 Oncogene (ECT2))

Autre désignation: ECT2 (ECT2 Produits)

Synonymes: anticorps ECT2, anticorps arhgef31, anticorps xect2, anticorps AI528536, anticorps mKIAA4037, anticorps ARHGEF31, anticorps ect2, anticorps epithelial cell transforming 2, anticorps ect2 oncogene, anticorps epithelial cell transforming 2 S homeolog, anticorps ECT2, anticorps ect2, anticorps Ect2, anticorps ect2.S

Sujet: ECT2, also known as epithelial cell transforming sequence 2 oncogene, was originally isolated as a transforming gene from epithelial cells. ECT2 catalyzes guanine nucleotide exchange on the small GTPases, RhoA, Rac1, and Cdc42. ECT2 may be phosphorylated during G2 and M phases, and phosphorylation may be required for its exchange activity. Unlike other known guanine nucleotide exchange factors for Rho GTPases, ECT2 exhibits nuclear localization in interphase, spreads throughout the cytoplasm in prometaphase, and is condensed in the midbody during cytokinesis. Expression of dominant-negative ECT2 or microinjection of affinity-purified anti-ECT2 antibody into interphase cells strongly inhibits cytokinesis. These results suggest that ECT2 is an important link between the cell cycle machinery and Rho signaling pathways involved in the regulation of cell division. Phosphorylation at T790 or S375 significantly affects the catalytic activity of ECT2.Synonyms: Epithelial cell-transforming sequence 2 oncogene

ID gène: 1894

NCBI Accession: NP_060568

UniProt: Q9H8V3

Pathways: Neurotrophin Signaling Pathway, Cell-Cell Junction Organization