NEDD4 anticorps (Internal Region)

N° du produit ABIN401453

Détail du produit anti-NEDD4 anticorps

Antigène: NEDD4 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4))

Épitope: Internal Region

Reactivité: Humain, Cheval, Macaque, Chien, Lapin, Chimpanzé

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NEDD4 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificité: This antibody reacts with endogenous Nedd4 protein.

Réactivité croisée (Details): Species reactivity (expected):Horse, Macaque, Chimpanzee, Rabbit, Canine, Cattle.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogène: Peptide corresponding to an internal portion of the Nedd4 protein

Isotype: IgG

Information d'application

Indications d'application: ELISA: 1/20,000 - 1/35,000. Western Blot: 1/300 to 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.64 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Détails sur NEDD4

Antigène: NEDD4 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase (NEDD4))

Autre désignation: NEDD4 (NEDD4 Produits)

Synonymes: anticorps NEDD4-1, anticorps RPF1, anticorps AA959633, anticorps AL023035, anticorps AU019897, anticorps E430025J12Rik, anticorps Nedd4-1, anticorps Nedd4a, anticorps mKIAA0093, anticorps CG32184, anticorps CG42279, anticorps CG7555, anticorps DNedd4, anticorps Dmel\\CG42279, anticorps Dmel_CG32184, anticorps Dmel_CG7555, anticorps dNedd4, anticorps dNedd4-1, anticorps nedd4, anticorps GB16853, anticorps NEDD4, anticorps Nedd4, anticorps neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase, anticorps neural precursor cell expressed, developmentally down-regulated 4, anticorps CG42279 gene product from transcript CG42279-RJ, anticorps E3 ubiquitin-protein ligase Nedd-4, anticorps E3 ubiquitin-protein ligase NEDD4, anticorps NEDD4, anticorps Nedd4, anticorps LOC411723, anticorps LOC100457150, anticorps nedd4, anticorps LOC100547485

Sujet: NEDD4 (neural precursor expressed, developmentally down-regulated protein 4, aliases: KIAA0093, MGC176705, NEDD4-1) is a gene that is highly expressed in early embryonic central nervous system. A family of NEDD4-like proteins has more recently been defined. NEDD4 and NEDD4-like proteins contain multiple functional domains including a calcium-dependent phospholipid and membrane binding domain (C2 domain), two to four protein binding domains (WW domains), and an E3 ubiquitin-protein ligase domain (HECT domain). NEDD4 and NEDD4-2 have been shown to down-regulate both neuronal voltage-gated Na+ channels (NaVs) and epithelial Na+ channels (ENaCs) in response to increased intracellular Na+ concentrations. The WW domains of NEDD4 bind to PY motifs (amino acid sequence PPXY) found in multiple NaV and ENaC proteins, and ubiquitination of these proteins, mediated by the HECT domain of NEDD4, results in their internalization and removal from the plasma membrane. Mutation of the PY motifs in ENaC proteins is associated with Liddle's Syndrome, an autosomal-dominant form of hypertension. In addition to targeting sodium channels, NEDD4-2 has also been shown to negatively regulate TGF-β signaling by targeting Smad2 for degradation. Mouse and human NEDD4 are cleaved by caspase proteins during apoptosis, although the significance of this cleavage is not clear.Synonyms: E3 ubiquitin-protein ligase NEDD4, KIAA0093

ID gène: 4734

NCBI Accession: NP_006145

UniProt: P46934

Pathways: Signalisation Notch, Intracellular Steroid Hormone Receptor Signaling Pathway, Skeletal Muscle Fiber Development, Signaling Events mediated by VEGFR1 and VEGFR2