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Ubiquitin anticorps Primary Antibody

Reactivité: Boeuf (Vache), Humain, Souris ELISA, IF, IHC, IHC (fro), IHC (p), WB Hôte: Souris Monoclonal
N° du produit ABIN462018
$727.83
Plus shipping costs $45.00
50 μg
local_shipping Destination: Etats-Unis
Envoi sous 11 à 14 jours ouvrables
  • Antigène
    Reactivité
    Boeuf (Vache), Humain, Souris
    Hôte
    Souris
    Clonalité
    Monoclonal
    Conjugué
    Inconjugué
    Application
    ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Marque
    IHC-plus™
    Specificité
    Recognizes bovine ubiquitin, both conjugated and unconjugated. Reacts with a single chain 8.5kD protein. The ubiquitin molecule appears to be present in all eukaryotic cells and has an identical primary structure in all animals. Species cross-reactivity: Human, chicken, mouse, C. elegans and Drosophila.
    Purification
    Protein A purified
    Immunogène
    Purified bovine ubiquitin conjugated to carrier protein.

    Type of Immunogen: Purified protein
    Isotype
    IgG1, kappa
  • Indications d'application
    Approved: ELISA (0.1 - 1 μg/mL), IF, IHC, IHC-Fr, IHC-P (10 μg/mL), WB

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: 1-3 μg/mL, Prepare lysis buffer in 10 mM N-ethylmaleimide to inhibit ubiquitin-conjugating enzymes. N-ethylmaleimide inactivates certain enzymes by blocking free sulfhydryls. After electrophoresis and transfer, pre-incubate transferred membranes in denaturing buffer (6M guanidine-HCl 20 mM Tris-HCl,  pH 7.5, 5 mM b-mercaptoethanol, 1 mM PMSF) for 30-60 minutes at 4uC, followed by extensive PBS washing.
    Commentaires

    Target Species of Antibody: Bovine

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS,  pH 7.4, 0.1 % sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Short term: 4°C
    Long term: Add glycerol (40-50%) -20°C.
  • Antigène
    Pathways
    Mitotic G1-G1/S Phases
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