Ubiquitin anticorps

Détails pour le produit réf. ABIN462018
Antigène
Reactivité
Boeuf (Vache), Humain, Souris
352
236
233
217
115
73
61
57
53
53
52
50
50
49
48
48
32
18
16
7
4
3
3
3
2
2
2
2
1
1
1
1
1
Hôte
Souris
271
241
5
5
3
1
Clonalité
Monoclonal
Conjugué
Inconjugué
21
18
17
16
16
16
11
11
11
11
11
11
11
11
11
11
11
8
7
7
7
7
7
3
3
3
3
3
3
1
1
1
1
1
Application
ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
469
320
214
151
134
98
69
56
22
19
16
13
12
5
1
1
1
Options
Marque IHC-plus™
Immunogène Purified bovine ubiquitin conjugated to carrier protein.

Type of Immunogen: Purified protein
Isotype IgG1, kappa
Specificité Recognizes bovine ubiquitin, both conjugated and unconjugated. Reacts with a single chain 8.5kD protein. The ubiquitin molecule appears to be present in all eukaryotic cells and has an identical primary structure in all animals. Species cross-reactivity: Human, chicken, mouse, C. elegans and Drosophila.
Purification Protein A purified
Antigène
Pathways Mitotic G1-G1/S Phases
Indications d'application Approved: ELISA (0.1 - 1 μg/mL), IF, IHC, IHC-Fr, IHC-P (10 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: 1-3 μg/mL, Prepare lysis buffer in 10 mM N-ethylmaleimide to inhibit ubiquitin-conjugating enzymes. N-ethylmaleimide inactivates certain enzymes by blocking free sulfhydryls. After electrophoresis and transfer, pre-incubate transferred membranes in denaturing buffer (6M guanidine-HCl 20 mM Tris-HCl,  pH 7.5, 5 mM b-mercaptoethanol, 1 mM PMSF) for 30-60 minutes at 4uC, followed by extensive PBS washing.
Commentaires

Target Species of Antibody: Bovine

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer PBS,  pH 7.4, 0.1 % sodium azide
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock 4 °C,-20 °C
Stockage commentaire Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
Images (Fournisseur)
Image no. 1 for anti-Ubiquitin antibody (ABIN462018) Human Brain, Cortex, Senile Plaque (formalin-fixed, paraffin-embedded) stained with U...
Image no. 2 for anti-Ubiquitin antibody (ABIN462018) Human Testis (formalin-fixed, paraffin-embedded) stained with Ubiquitin antibody ABIN...
Image no. 3 for anti-Ubiquitin antibody (ABIN462018) Anti-Ubiquitin antibody IHC of human brain, cortex, senile plaque. Immunohistochemist...