APOB anticorps

N° du produit ABIN462332

Détail du produit anti-APOB anticorps

Antigène: APOB (Apolipoprotein B (APOB))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp APOB est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Typically less than 1 % cross reactivity againstother types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein B and has negligible cross-reactivity with Type A-I, A-II, C-I, C-II, C-III, E and J apoLipoproteins. Specific cross reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible

Purification: Immunoaffinity purified

Immunogène: Apolipoprotein Type B was isolated from human plasma by density gradient centrifugation followed by HPLC purification.

Information d'application

Indications d'application: Approved: ELISA (1:2000 - 1:10000), IHC, IHC-P (5 μg/mL), IP, WB (1:2000 - 1:10000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.125 M sodium borate, 0.075 M sodium chloride, 0.005 M EDTA,  pH 8.0, 0.01 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4°C prior to opening. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below.

Détails sur APOB

Antigène: APOB (Apolipoprotein B (APOB))

Autre désignation: Apolipoprotein B / APOB (APOB Produits)

Synonymes: anticorps FLDB, anticorps LDLCQ4, anticorps Ac1-060, anticorps Apo B-100, anticorps ApoB-100, anticorps ApoB-48, anticorps AI315052, anticorps apob-100, anticorps apob-48, anticorps APOB-100, anticorps ApoB(100), anticorps APOB, anticorps apolipoprotein B, anticorps APOB, anticorps Apob

Sujet: Name/Gene ID: APOB

Synonyms: APOB, ApoB-100, Apolipoprotein B-48, ApoB-48, Apolipoprotein B, FLDB, LDLCQ4, Mutant Apo B 100, Apo B-100, Apolipoprotein B-100, Apolipoprotein B48

ID gène: 338

UniProt: P04114

Pathways: Lipid Metabolism