IL-6 anticorps (Biotin)

N° du produit ABIN465328

Cet anticorps anti-IL-6 est un anticorps Chèvre Polyclonal détectant IL-6 dans WB, ELISA et Neut. Adapté pour Humain.

Aperçu rapide pour IL-6 anticorps (Biotin) (ABIN465328)

Antigène: IL-6 (IL6) (Interleukin 6 (IL6))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp IL-6 est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Détail du produit anti-IL-6 anticorps (Biotin)

Specificité: Human IL-6

Purification: Immunoaffinity purified

Immunogène: E.coli derived recombinant Human IL-6.

Type of Immunogen: Cell extract

Information d'application

Indications d'application: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect Human IL-6 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-6. To detect Human IL-6 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IL-6 (LS-C104544) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-6. Western Blot: To detect Human IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-6 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Conseil sur la manipulation: Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Détails sur IL-6

Antigène: IL-6 (IL6) (Interleukin 6 (IL6))

Autre désignation: IL6

Sujet: Name/Gene ID: IL6
Family: Hormone

Synonyms: IL6, B-cell stimulatory factor 2, BSF-2, BSF2, CTL differentiation factor, Hybridoma growth factor, IFN-beta-2, Interleukin-6, Interferon beta-2, Interleukin BSF-2, IFNB2, B-cell differentiation factor, CDF, HSF, IL-6

ID gène: 3569

UniProt: P05231

Pathways: Signalisation TLR, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome