Leptin anticorps

N° du produit ABIN465758

Détail du produit anti-Leptin anticorps

Antigène: Leptin (LEP)

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Leptin est non-conjugé

Application: Western Blotting (WB), ELISA

Specificité: Mouse Leptin

Purification: Immunoaffinity purified

Immunogène: E.coli derived recombinant Murine Leptin.

Type of Immunogen: Cell extract

Information d'application

Indications d'application: Approved: ELISA, WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody stained 4 % PFA, paraffin-embedded sections of Trichuris muris infected murine tissue. The primary antibody was incubated at 0.20 μg/mL overnight at 4 °C and the secondary antibody was a cyanine-3 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100  mM citric acid was used. Information and photo are courtesy of David Artis, University of Pennsylvania. Optimal concentrations and conditions may vary. ELISA: To detect mLeptin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine Leptin (LS-C104759) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mLeptin. Western Blot: To detect mLeptin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mLeptin is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Commentaires:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile ddH2O.

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Conseil sur la manipulation: Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Détails sur Leptin

Antigène: Leptin (LEP)

Autre désignation: Leptin (LEP Produits)

Synonymes: anticorps ob, anticorps obese, anticorps LEPD, anticorps OB, anticorps OBS, anticorps leptin, anticorps Lep, anticorps LEP, anticorps lep

Sujet: Name/Gene ID: LEP

Synonyms: LEP, Leptin, LEPD, Obese protein, Obese, mouse, homolog of, Obesity factor, OBS, OB

ID gène: 3952

UniProt: P41159

Pathways: Signalistation JAK/STAT, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process