Rhodopsin anticorps (C-Term)

N° du produit ABIN487512

Détail du produit anti-Rhodopsin anticorps

Antigène: Rhodopsin (RHO)

Épitope: C-Term

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Rhodopsin est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Specificité: Recognizes Rhodopsin (~40? kDa). Reacts with both native and recombinant protein. No known reactivity to other proteins. The antibody specifically binds to the C-terminal epitope -T-E-T-S-Q-V-A-P-A- (COOH) of Rhodopsin. Proteins containing the epitope tag can be selectively bound to a Rho 1D4 affinity matrix and eluted using an excess of -T-E-T-S-Q-V-A-P-A- peptide under mild conditions.

Réactivité croisée (Details): Species reactivity (tested):Vertebrates.

Attributs du produit: Synonyms: RHO, Opsin-2, OPN2

Purification: Protein G Chromatography

Immunogène: Bovine Rhodopsin.

Clone: 1D4

Isotype: IgG1

Information d'application

Indications d'application: ELISA. Western blot: 1/1000 (1)1 μg/mL was sufficient for detection of Rhodopsin in 10 μg of Rat eye lysate by colorimetricimmunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody. Immunoprecipitation: 1-2 μg per 100-500 μg of proteinImmunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS pH 7.4, 0.09 % Sodium Azide, 50 % Glycerol

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur Rhodopsin

Antigène: Rhodopsin (RHO)

Autre désignation: Rhodopsin (RHO Produits)

Synonymes: anticorps CSNBAD1, anticorps OPN2, anticorps RP4, anticorps RH1, anticorps Rh, anticorps fi06d11, anticorps wu:fi06d11, anticorps zfo2, anticorps zfrho, anticorps Noerg1, anticorps Opn2, anticorps Ops, anticorps opn2, anticorps rh1, anticorps rp4, anticorps xrho, anticorps PIGRHO1, anticorps ops, anticorps RDP1, anticorps RHO, anticorps rhodopsin, anticorps rhodopsin L homeolog, anticorps RHO, anticorps Rho, anticorps rho, anticorps rho.L

Classe de substances: Chemical

Sujet: Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photoreactive chromophore, in a central pocket (Ref.2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).Synonyms: OPN2, Opsin-2, RHO

Poids moléculaire: 48 kDa

ID gène: 6010

UniProt: P08100

Pathways: Signalisation WNT, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction