ARFGAP1 anticorps (AA 1-183)

N° du produit ABIN4886474

L’anticorps Lapin Polyclonal anti-ARFGAP1 a été validé pour WB, IHC, IF, FACS et ICC. Il convient pour détecter ARFGAP1 dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour ARFGAP1 anticorps (AA 1-183) (ABIN4886474)

Antigène: ARFGAP1 (ADP-Ribosylation Factor GTPase Activating Protein 1 (ARFGAP1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ARFGAP1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Détail du produit anti-ARFGAP1 anticorps

Épitope: AA 1-183

Fonction: Anti-ARFGAP1 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-ARFGAP1 Antibody Picoband® (ABIN4886474). Tested in Flow Cytometry, ICC, IHC, IF, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E. coli-derived human ARFGAP1 recombinant protein (Position: M1-Y183). Human ARFGAP1 shares 89.1% and 88.5% amino acid (aa) sequence identity with mouse and rat ARFGAP1, respectively.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. "Entrez Gene: ARFGAP1 ADP-ribosylation factor GTPase activating protein 1". 2. Antonny, Bruno (June 2011). "Mechanisms of membrane curvature sensing". Annu Rev Biochem. 80: 101-123. 3. Huber I, Rotman M, Pick E, Makler V, Rothem L, Cukierman E, Cassel D (February 2001). "Expression, purification, and properties of ADP-ribosylation factor (ARF) GTPase activating protein-1". Methods Enzymol. 329: 307-16.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur ARFGAP1

Antigène: ARFGAP1 (ADP-Ribosylation Factor GTPase Activating Protein 1 (ARFGAP1))

Autre désignation: ARFGAP1

Sujet:

Synonyms: ADP-ribosylation factor GTPase-activating protein 1,ARF GAP 1,ADP-ribosylation factor 1 GTPase-activating protein,ARF1 GAP,ARF1-ed GTPase-activating protein,ARFGAP1,ARF1GAP,

Tissue Specificity: Ubiquitous. .

Background: ADP-ribosylation factor GTPase-activating protein 1 is an enzyme that in humans is encoded by the ARFGAP1 gene. And this gene is mapped to 20q13.33. The protein encoded by this gene is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Alternative splicing results in multiple transcript variants.

Poids moléculaire: 45 kDa

ID gène: 55738

UniProt: Q8N6T3

Pathways: Signalisation p53, ER-Nucleus Signaling, Unfolded Protein Response