NF-kB p65 anticorps (C-Term)

N° du produit ABIN4889656

Il existe 3+ publications pour ce produit. L’anticorps anti-NF-kB p65 Polyclonal Lapin est utilisé pour la détection de NF-kB p65 dans des échantillons de Humain. Il a été validé pour WB, ChIP, ChIP-seq et SSA.

Aperçu rapide pour NF-kB p65 anticorps (C-Term) (ABIN4889656)

Antigène: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NF-kB p65 est non-conjugé

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq), Supershift Assay (SSA)

Détail du produit anti-NF-kB p65 anticorps

Épitope: C-Term

Fonction: NFkB p65 antibody (pAb)

Purification: Unpurified

Immunogène: This NFkB p65 antibody was raised against a peptide immunogen corresponding to the C-terminal of human p65.

Information d'application

Indications d'application: ChIP-Seq: 10 µL per ChIP ChIP: 4 µL per ChIP WB: 1:2,500 - 1:5,000 dilution

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-NF-kB p65 (ABIN4889656)

Diermeier, Kolovos, Heizinger, Schwartz, Georgomanolis, Zirkel, Wedemann, Grosveld, Knoch, Merkl, Cook, Längst, Papantonis: "TNF? signalling primes chromatin for NF-?B binding and induces rapid and widespread nucleosome repositioning." dans: Genome biology, Vol. 15, Issue 12, pp. 536, (2015) (PubMed).

Raaz, Schellinger, Chernogubova, Warnecke, Kayama, Penov, Hennigs, Salomons, Eken, Emrich, Zheng, Adam, Jagger, Nakagami, Toh, Toyama, Deng, Buerke, Maegdefessel, Hasenfuß, Spin, Tsao: "Transcription Factor Runx2 Promotes Aortic Fibrosis and Stiffness in Type 2 Diabetes Mellitus." dans: Circulation research, Vol. 117, Issue 6, pp. 513-24, (2015) (PubMed).

Anderson, Rahmutula, Gardner: "Tumor necrosis factor-alpha inhibits endothelial nitric-oxide synthase gene promoter activity in bovine aortic endothelial cells." dans: The Journal of biological chemistry, Vol. 279, Issue 2, pp. 963-9, (2004) (PubMed).

Détails sur NF-kB p65

Antigène: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Autre désignation: NFkappaB p65

Sujet: NFkB p65 (Nuclear factor NF-kappa-B p65 subunit also known as NFkB p65 or RelA) is a member of the Rel family of transcription factors that are involved in processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. The Rel family includes c-Rel, RelA (NFkB p65, NFkB3) and RelB (I-Rel). Rel proteins form dimers with the NFkB p50 (proteolytically processed from p105/NFkB1) and p52 (proteolytically processed from p100/NFkB2) members of the NFkB family that bind DNA and activate transcription. The NFkB p65/p50 heterodimer is the most abundant form of NFkB. NFkB signaling is negatively regulated by the sequestration of the NFkB complex in the cytoplasm by its association with the IkB family of inhibitory proteins. The IkB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFkB. The IKK complex is composed of two catalytic subunits (IKKa and IKKb) and a regulatory subunit (IKKg). Upon its activation, the IKK complex phosphorylates IkB proteins (IkBa, IkBa phospho Ser32, 36), which marks them for ubiquitination and degradation. This enables the NFkB complex to translocate to the nucleus where it regulates transcription by binding to DNA.

Poids moléculaire: 65 kDa

ID gène: 5970

NCBI Accession: NP_068810

Pathways: Signalisation NF-kappaB, Signalisation RTK, TCR Signaling, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins