c-MET anticorps (Activation Domain)

N° du produit ABIN492622

Cet anticorps Souris Monoclonal détecte spécifiquement c-MET dans WB. Il présente une réactivité envers Humain.

Aperçu rapide pour c-MET anticorps (Activation Domain) (ABIN492622)

Antigène: c-MET (MET) (Met Proto-Oncogene (MET))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp c-MET est non-conjugé

Application: Western Blotting (WB)

Clone: 14G9

Détail du produit anti-c-MET anticorps

Épitope: Activation Domain

Specificité: This monoclonal MET antibody (14G9) recognizes Met activation loop.

Attributs du produit: Synonyms: Hepatocyte growth factor receptor, MET, Scatter factor receptor, HGF/SF receptor, c-Met,Met proto-oncogene tyrosine kinase

Purification: Subsequent Ultrafiltration and Size Exclusion Chromatography.

Ingrédients: incl. positive Control

Immunogène: peptide conjugated to hemocyaninEpitope: kinase activation loop.

Isotype: IgG1

Information d'application

Indications d'application: Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated A431 cells (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: Positive Control Provided. Cell lysate from untreated A431 cellsDescription: Cell lysate from untreated A431 cells, epidermoid carcinoma (Human)Format: Lyophilized cell lysate from serum starved A431 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mLPBS, containing 0.09 % Sodium Azide/PEG and Sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Avoid repeated freeze / thaw cycles. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.

Date de péremption: 12 months

Détails sur c-MET

Antigène: c-MET (MET) (Met Proto-Oncogene (MET))

Autre désignation: HGF Receptor

Sujet: MET is a high affinity receptor for Hepatocyte Growth Factor/Scatter Factor HGF/SF. The (HGF/SF)-(HGFR/MET) ligand-receptor system appears to have an important role in controlling interaction between mesenchymal cells and other cell types and has been implicated in the cell mitogenic response, cell motility and development. The interaction with HGF leads to autophosphorylation at multiple tyrosine residues and recruiting of several downstream signaling components (Gab1, c-cbl, PI3-kinase). The phosphorylation of Tyr 1234/1235 leads to kinase activation, and phospho-Tyr 1349 is the Gab1 binding site. Altered MET levels and/or tyrosine kinase activities have been found in different tumors, such as colon, renal, and breast cancer.Synonyms: HGF/SF receptor, Hepatocyte growth factor receptor, MET, Met proto-oncogene tyrosine kinase, Scatter factor receptor, c-Met

ID gène: 4233

UniProt: P08581

Pathways: Signalisation RTK, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor