GSK3 beta anticorps

N° du produit ABIN4953256

L’anticorps anti-GSK3 beta Monoclonal produit chez le Souris a été validé pour WB, IF, IP et ICC. Il convient pour la détection de GSK3 beta dans des échantillons provenant de Humain, Souris et Rat. }

Aperçu rapide pour GSK3 beta anticorps (ABIN4953256)

Antigène: GSK3 beta (GSK3b) (Glycogen Synthase Kinase 3 beta (GSK3b))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GSK3 beta est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 2E6-D6-C12

Détail du produit anti-GSK3 beta anticorps

Purification: Protein G affinity

Immunogène: A recombinant partial protein was used as the immunogen for this GSK3B antibody.

Isotype: IgG2a

Information d'application

Indications d'application: The stated application concentrations are suggested starting points. Titration of the GSK3B antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,ICC/IF: 1:200,Immunoprecipitation: suitable

Restrictions: For Research Use only

Stockage

Buffer: Antibody in PBS with 50 % Glycerol and 0.03 % ProClin 300

Stock: -20 °C

Stockage commentaire: Store the GSK3B antibody at -20°C.

Détails sur GSK3 beta

Antigène: GSK3 beta (GSK3b) (Glycogen Synthase Kinase 3 beta (GSK3b))

Autre désignation: GSK3 beta / GSK3B

Sujet: Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. Requires primed phosphorylation of the majority of its substrates. In skeletal muscle, contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. May also mediate the development of insulin resistance by regulating activation of transcription factors. Regulates protein synthesis by controlling the activity of initiation factor 2B (EIF2BE/EIF2B5) in the same manner as glycogen synthase. In Wnt signaling, GSK3B forms a multimeric complex with APC, AXIN1 and CTNNB1/beta-catenin and phosphorylates the N-terminus of CTNNB1 leading to its degradation mediated by ubiquitin/proteasomes. [UniProt]

UniProt: P49841

Pathways: Signalisation WNT, Signalisation Hedgehog, Fc-epsilon Receptor Signaling Pathway, Cellular Glucan Metabolic Process, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Autophagy, BCR Signaling, L'effet Warburg