BAD anticorps (pSer99)

N° du produit ABIN3030130

Cet anticorps Lapin Polyclonal détecte spécifiquement BAD dans ELISA, IHC, DB et IF. Il présente une réactivité envers Humain.

Aperçu rapide pour BAD anticorps (pSer99) (ABIN3030130)

Antigène: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp BAD est non-conjugé

Application: ELISA, Immunohistochemistry (IHC), Dot Blot (DB), Immunofluorescence (IF)

Détail du produit anti-BAD anticorps

Épitope: pSer99

Réactivité croisée (Details): Expected species reactivity: Mouse, Rat

Purification: Antigen affinity purified

Immunogène: This phospho-Bad antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS99 of human Bad.

Isotype: Ig Fraction

Information d'application

Indications d'application: Titration of the phospho-Bad antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunofluorescence: 1:200,Dot blot: 1:500,IHC (Paraffin): 1:50-1:100

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot the phospho-Bad antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Détails sur BAD

Antigène: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Autre désignation: Bad

Sujet: Bad is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin are found to be involved in the regulation of this protein. Bad is phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation.

UniProt: Q92934

Pathways: Signalisation MAPK, Signalisation PI3K-Akt, Signalisation RTK, Apoptose, Fc-epsilon Receptor Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events