ZC3HC1 anticorps

N° du produit ABIN498264

Détail du produit anti-ZC3HC1 anticorps

Antigène: ZC3HC1 (Zinc Finger, C3HC-Type Containing 1 (ZC3HC1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ZC3HC1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Specificité: This antibody detects endogenous levels of NIPA protein.

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity-Chromatography using epitope-specific immunogen

Information d'application

Indications d'application: Western blot: 1: 500 - 1: 1000. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS pH ~7.2 with 0.05 % Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur ZC3HC1

Antigène: ZC3HC1 (Zinc Finger, C3HC-Type Containing 1 (ZC3HC1))

Autre désignation: ZC3HC1 (ZC3HC1 Produits)

Synonymes: anticorps 1110054L24Rik, anticorps AU018540, anticorps AU019789, anticorps HSPC216, anticorps Nipa, anticorps NIPA, anticorps RGD1561099, anticorps zinc finger, C3HC type 1, anticorps zinc finger C3HC-type containing 1, anticorps zinc finger, C3HC-type containing 1, anticorps Zc3hc1, anticorps ZC3HC1

Sujet: Entry into mitosis is essentially driven by cyclin B1, which is located in the cytoplasm throughout interphase, but accumulates in the nucleus just before mitosis occurs. Nuclear interaction partner of ALK (NIPA) plays a critical role in cyclin B1 regulation. NIPA is normally phosphorylated during G2 and M phases, resulting in an accumulation of cyclin B1. When NIPA sheds its attached phosphate, it binds to SCF to form the SCFNIPA complex, a member of the E3 ubiquitin ligases, which ubiquitinates cyclin B1, thereby targeting it to the proteosome for degradation. Therefore, the accumulation of cyclin B1 is due to the inability of phosphorylated NIPA to bind to the molecule SCF, thereby preventing the degradation of cyclin B1. An absence of NIPA causes cyclin B1 to accumulate abnormally, leading to premature mitotic entry, loss of checkpoint control and genomic instability, which are all associated with cancer. The phosphorylated form of NIPA may also be involved in apoptotic signaling pathways.Synonyms: HSPC216, NIPA, Nuclear-interacting partner of anaplastic lymphoma kinase (ALK), Zinc finger C3HC-type protein 1

Poids moléculaire: approx. 55 kDa

ID gène: 51530

NCBI Accession: NP_057562

UniProt: Q86WB0