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Histone 3 anticorps (AA 1-50)

Name: Histone 3 anticorps (AA 1-50)
SKU: ABIN5004684
Price: 502.31 EUR
Availability: InStock

H3 Reactivité: Humain, Souris, Rat WB, IHC (p), IF (p) Hôte: Souris Monoclonal 3G1 unconjugated

K562 cell lysates; probed with Histone H3/HIST3H3 (3G1) Monoclonal Antibody, unconjugated (bsm-33042M) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C. (Histone 3 anticorps (AA 1-50))

Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Histone H3/HIST3H3 (3G1) Monoclonal Antibody, Unconjugated (bsm-33042M)at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining. (Histone 3 anticorps (AA 1-50))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Histone H3/HIST3H3 (3G1) Monoclonal Antibody, Unconjugated (bsm-33042M)at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining. (Histone 3 anticorps (AA 1-50))

IHC (p)

HeLa cell lysates; probed with Histone H3/HIST3H3 (3G1) Monoclonal Antibody, unconjugated (bsm-33042M) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C. (Histone 3 anticorps (AA 1-50))

4 images

(2 références)

N° du produit ABIN5004684

Fiche de données

Antigène Voir toutes Histone 3 (H3) Anticorps

Histone 3 (H3)
Épitope
60

47

44

44

41

36

35

35

34

34

34

32

29

29

28

27

26

25

23

22

22

21

20

18

18

17

16

16

16

16

16

16

15

14

14

13

13

12

12

12

11

11

11

10

10

10

10

9

9

8
AA 1-50
Reactivité
1376

939

860

59

42

41

41

40

39

35

35

30

26

22

19

6

3

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Humain, Souris, Rat
Hôte
1184

245

12

4
Souris
Anticorps secondaires appropriés
Clonalité
1109

335

1
Monoclonal
Conjugué
797

81

65

64

63

63

63

62

34

33

22

17

17

17

17

17

12

1
Cet anticorp Histone 3 est non-conjugé
Application
1034

402

373

311

240

188

175

171

162

151

137

127

116

48

38

34

32

20

16

14

7

7

6

4

3

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Réactivité croisée

Humain, Souris, Rat

Purification

Purified by Protein G.

Immunogène

Recombinant human HIST3H3 Protein

Clone

3G1

Isotype

IgG

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H3 Reactivité: Humain, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN Hôte: Lapin Polyclonal unconjugated

anti-Histone 3 (H3) (H3K36me3) antibody
H3 Reactivité: Humain, Souris WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag Hôte: Souris Monoclonal MABI 0333 unconjugated

anti-Histone 3 (H3) (H3K9ac) antibody
H3 Reactivité: Humain, Souris WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Hôte: Lapin Polyclonal unconjugated

anti-Histone 3 (H3) (AA 71-136) antibody
H3 Reactivité: Humain, Souris, Rat WB, ELISA, ICC, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

anti-Histone 3 (H3) (H3K27me) antibody
H3 Reactivité: Humain WB, IHC, IF, ChIP, IP, ChIP-seq Hôte: Lapin Polyclonal unconjugated

anti-Histone 3 (H3) (H3K4me3) antibody
H3 Reactivité: Humain, Souris, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Hôte: Lapin Polyclonal unconjugated

anti-Histone 3 (H3) (C-Term) antibody
H3 Reactivité: Humain WB, IHC, ChIP, IP Hôte: Lapin Polyclonal unconjugated

anti-Histone 3 (H3) (H3K4me3) antibody
H3 Reactivité: Humain WB, IHC, IF, ChIP, IP, DB, ChIP-seq, CUT&RUN, CUT&Tag Hôte: Lapin Polyclonal unconjugated

Indications d'application

WB 1:300-5000
IHC-P 1:200-400
IF(IHC-P) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur

ProClin

Précaution d'utilisation

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock

4 °C,-20 °C

Stockage commentaire

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption

12 months
Tian, Wu, Guo, Ma, Huang, Zhao: "Minocycline attenuates sevoflurane-induced cell injury via activation of Nrf2." dans: International journal of molecular medicine, Vol. 39, Issue 4, pp. 869-878, (2017) (PubMed).

Zhao, Ding, Liu, Yin, Zhang, Ma: "Unfractionated heparin protects the protein C system against lipopolysaccharide-induced damage in vivo and in vitro." dans: Experimental and therapeutic medicine, Vol. 14, Issue 6, pp. 5515-5522, (2017) (PubMed).
Antigène

Histone 3 (H3)

Autre désignation

Histone H3 (H3 Produits)

Synonymes

anticorps H-3, anticorps histocompatibility 3, anticorps H3

Sujet

Synonyms: Histone Cluster 3, H3, H3 Histone Family, Member T, Histone 3, H3, H3FT, H3/G, H3/T, H3t, H3.4 , Histone H3.1t , HIST3H3, HGNC:4778, H31T_HUMAN
Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]

ID gène

8290

UniProt

Q16695