PARP1 anticorps (AA 373-540)

N° du produit ABIN5541684

L’anticorps Souris Monoclonal anti-PARP1 a été validé pour WB et IHC (p). Il convient pour détecter PARP1 dans des échantillons de Humain.

Aperçu rapide pour PARP1 anticorps (AA 373-540) (ABIN5541684)

Antigène: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PARP1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 839120

Détail du produit anti-PARP1 anticorps

Épitope: AA 373-540

Specificité: The antibody recognizes Human PARP. Other species not tested.

Purification: Affinity Chromatography on Protein A/G

Immunogène: E.coli -derived recombinant human PARP- Thr 373-Glu 540

Isotype: IgG1

Information d'application

Indications d'application: Western Blot: 1 μg/mL. Positive Control : HeLa cell lysate. Immunohistochemistry on Paraffin Sections: 8-25 μg/mL. Positive Control: Human breast tissue.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Sterile PBS

Stock: -20 °C

Stockage commentaire: Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur PARP1

Antigène: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Autre désignation: parp1

Sujet: PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress. This protein can be cleaved by many ICE-like Caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa). PARP helps cells to maintain their viability cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis.

UniProt: P09874

Pathways: Apoptose, Caspase Cascade in Apoptosis, Réparation de l'ADN, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location